MetaCyc Pathway: glutaminyl-tRNA<sup>gln</sup> biosynthesis via transamidation in Sphingobium sp. HT1-2

MetaCyc Pathway: glutaminyl-tRNA^gln biosynthesis via transamidation in Sphingobium sp. HT1-2

Glutamate synthase (NADPH):
L-glutamine + H₂O→ammonia + L-glutamate + H⁺ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)

Glutamate--tRNA(Gln) ligase:
L-glutamate + ATP + a tRNA^Gln→an L-glutamyl-[tRNA^Gln] + AMP + diphosphate (EC 6.1.1.24)

Glutaminyl-tRNA synthase (glutamine-hydrolyzing):
an L-glutamyl-[tRNA^Gln] + ATP→a 5-phosphooxy-L-glutamyl-[tRNA^Gln] + ADP (EC 6.3.5.7)

Glutaminyl-tRNA synthase (glutamine-hydrolyzing):
a 5-phosphooxy-L-glutamyl-[tRNA^Gln] + ammonia→an L-glutaminyl-[tRNA^Gln] + phosphate (EC 6.3.5.7)

Links:

Pathway details at MetaCyc
Fitness data for 18 genes
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All MetaCyc pathways for Sphingobium sp. HT1-2

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information