MetaCyc Pathway: L-glutamate degradation VII (to butanoate) in Sphingobium sp. HT1-2

MetaCyc Pathway: L-glutamate degradation VII (to butanoate) in Sphingobium sp. HT1-2

Methylaspartate mutase (in reverse):
L-glutamate→(2S, 3S)-3-methylaspartate (EC 5.4.99.1)

No genes

Methylaspartate ammonia-lyase:
(2S, 3S)-3-methylaspartate→mesaconate + ammonium (EC 4.3.1.2)

No genes

(S)-2-methylmalate dehydratase (in reverse):
mesaconate + H₂O→(S)-citramalate (EC 4.2.1.34)

No genes

Citramalate lyase:
(S)-citramalate→pyruvate + acetate (EC 4.1.3.22)

No genes

Pyruvate synthase:
pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + 2 a reduced ferredoxin [iron-sulfur] cluster + CO₂ + H⁺ (EC 1.2.7.1)

No genes

Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)

Ferredoxin hydrogenase (in reverse):
2 a reduced ferredoxin [iron-sulfur] cluster + 2 H⁺→H₂ + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.12.7.2)

3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H⁺→(S)-3-hydroxybutanoyl-CoA + NAD⁺ (EC 1.1.1.35)

(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H₂O (EC 4.2.1.150)

crotonyl-CoA_[cytosol] + 2 NADH_[cytosol] + 2 an oxidized ferredoxin [iron-sulfur] cluster_[cytosol]→2 a reduced ferredoxin [iron-sulfur] cluster_[cytosol] + butanoyl-CoA_[cytosol] + 2 NAD⁺_[cytosol] (EC 1.3.1.109)

No genes

Phosphate butyryltransferase:
butanoyl-CoA + phosphate→butanoyl phosphate + coenzyme A (EC 2.3.1.19)

Branched-chain-fatty-acid kinase (in reverse):
butanoyl phosphate + ADP→butanoate + ATP (EC 2.7.2.14; 2.7.2.7)

No genes

Links:

Pathway details at MetaCyc
Fitness data for 35 genes
Select organism:
All MetaCyc pathways for Sphingobium sp. HT1-2

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information