MetaCyc Pathway: pyruvate fermentation to isobutanol (engineered) in Ralstonia solanacearum UW163

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Acetolactate synthase:
2 pyruvate + H+→(S)-2-acetolactate + CO2
(EC 2.2.1.6)
UW163_RS10675
UW163_RS10680
UW163_RS13455
Ketol-acid reductoisomerase (in reverse):
(S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+
(EC 1.1.1.86)
UW163_RS10685
Dihydroxy-acid dehydratase:
(2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O
(EC 4.2.1.9)
UW163_RS09135
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2
(EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+
(EC 1.1.1.1)
UW163_RS01260
UW163_RS05800
UW163_RS10540
UW163_RS15560
UW163_RS17175
UW163_RS21255
UW163_RS22615
UW163_RS22665

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information