MetaCyc Pathway: phenylacetate degradation I (aerobic) in Pseudomonas putida KT2440

Phenylacetate--CoA ligase: phenylacetate + ATP + coenzyme A→phenylacetyl-CoA + AMP + diphosphate (EC 6.2.1.30)

PP_3279

phenylacetyl-CoA + NADPH + dioxygen + H+→2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA + NADP+ + H2O (EC 1.14.13.149)

PP_3274

PP_3276

PP_3277

PP_3278

2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA→2-oxepin-2(3H)-ylideneacetyl-CoA (EC 5.3.3.18)

PP_3283

2-oxepin-2(3H)-ylideneacetyl-CoA + H2O→3-oxo-5,6-didehydrosuberyl-CoA semialdehyde (EC 3.3.2.12)

PP_3270

3-oxo-5,6-didehydrosuberyl-CoA semialdehyde + NADP+ + H2O→3-oxo-5,6-didehydrosuberyl-CoA + NADPH + 2 H+ (EC 1.2.1.91)

PP_3270

3-oxo-5,6-didehydrosuberyl-CoA + coenzyme A→(2E)-5-carboxy-2-pentenoyl-CoA + acetyl-CoA (EC 2.3.1.223)

PP_3280

Enoyl-CoA hydratase (in reverse): (2E)-5-carboxy-2-pentenoyl-CoA + H2O→(3S)-hydroxyadipyl-CoA (EC 4.2.1.17)

PP_1412

PP_1845

PP_2047

PP_2136

PP_2217

PP_3283

PP_3284

PP_3358

PP_3491

PP_3726

PP_3732

PP_4030

3-hydroxyacyl-CoA dehydrogenase: (3S)-hydroxyadipyl-CoA + NAD+→3-oxoadipoyl-CoA + NADH + H+ (EC 1.1.1.35)

PP_2047

PP_2136

3-oxoadipyl-CoA thiolase (in reverse): 3-oxoadipoyl-CoA + coenzyme A→succinyl-CoA + acetyl-CoA (EC 2.3.1.174)

PP_1377

PP_3280

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information