MetaCyc Pathway: superpathway of tetracycline and oxytetracycline biosynthesis in Pseudomonas putida KT2440

Add experiment(s):


malonyl-CoA + ATP + L-glutamine + H2O→malonamoyl-CoA + AMP + L-glutamate + diphosphate + H+ (EC 6.3.5.M2)
No genes
malonamoyl-CoA + an OxyC acyl-carrier protein→malonamoyl-[OxyC acyl-carrier protein] + coenzyme A (EC 2.3.1.M29)
No genes
8 malonyl-CoA + malonamoyl-[OxyC acyl-carrier protein] + 8 H+→nonaketamide-[OxyC acyl-carrier protein] + 8 CO2 + 8 coenzyme A (EC 2.3.1.260)
No genes
nonaketamide-[OxyC acyl-carrier protein] + NADPH + H+→C9-reduced nonaketamide-[OxyC acyl-carrier protein] + NADP+ (EC 1.1.1.M31)
No genes
C9-reduced nonaketamide-[OxyC acyl-carrier protein]→nonaketamide monocyclic intermediate-[OxyC acyl-carrier protein] + 2 H2O (EC 4.2.1.M9)
No genes
nonaketamide monocyclic intermediate-[OxyC acyl-carrier protein]→nonaketamide tricyclic intermediate-[OxyC acyl-carrier protein] + 2 H2O (EC 4.2.1.M10)
PP_3207
nonaketamide tricyclic intermediate-[OxyC acyl-carrier protein]→pretetramide + an OxyC acyl-carrier protein + H+
No genes
pretetramide + S-adenosyl-L-methionine→6-methylpretetramide + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.M31)
No genes
6-methylpretetramide + NADPH + dioxygen + H+→4-hydroxy-6-methylpretetramide + NADP+ + H2O (EC 1.14.13.232)
No genes
4-hydroxy-6-methylpretetramide + NADPH + dioxygen + H+→4-dedimethylamino-4-oxoanhydrotetracycline + NADP+ + H2O (EC 1.14.13.233)
No genes
4-dedimethylamino-4-oxoanhydrotetracycline + an aminated amino group donor→4-amino-4-de(dimethylamino)anhydrotetracycline + a deaminated amino group donor (EC 2.6.1.M2)
No genes
4-amino-4-de(dimethylamino)anhydrotetracycline + S-adenosyl-L-methionine→4-methylamino-4-de(dimethylamino)anhydrotetracycline + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.335)
No genes
4-methylamino-4-de(dimethylamino)anhydrotetracycline + S-adenosyl-L-methionine→anhydrotetracycline + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.335)
No genes
Anhydrotetracycline monooxygenase:
anhydrotetracycline + NADPH + dioxygen + H+→5a,11a-dehydrotetracycline + NADP+ + H2O (EC 1.14.13.38)
No genes
5a,11a-dehydrotetracycline + NADPH + dioxygen + H+→5a,11a-dehydrooxytetracycline + NADP+ + H2O (EC 1.14.13.234)
No genes
5a,11a-dehydrotetracycline + a reduced factor 420→tetracycline + an oxidized factor 420 (EC 1.3.98.4)
No genes
5a,11a-dehydrooxytetracycline + a reduced factor 420→oxytetracycline + an oxidized factor 420 (EC 1.3.98.4)
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information