MetaCyc Pathway: 2-methylpropene degradation in Pseudomonas putida KT2440

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Alkene monooxygenase:
2-methylprop-1-ene + NADH + dioxygen + H+→1,2-epoxy-2-methylpropane + NAD+ + H2O (EC 1.14.13.69)
No genes
Soluble epoxide hydrolase:
1,2-epoxy-2-methylpropane + H2O→2-methylpropane-1,2-diol (EC 3.3.2.10)
No genes
2-methylpropane-1,2-diol + NAD+→2-hydroxy-2-methylpropanal + NADH + H+ (EC 1.1.1.400)
No genes
2-hydroxy-2-methylpropanal + NAD+ + H2O→2-hydroxy-2-methylpropanoate + NADH + 2 H+ (EC 1.2.1.98)
No genes
2-hydroxy-2-methylpropanoate + ATP + coenzyme A→2-hydroxy-2-methylpropanoyl-CoA + AMP + diphosphate (EC 6.2.1.M4)
No genes
2-hydroxy-2-methylpropanoyl-CoA→(S)-3-hydroxybutanoyl-CoA (EC 5.4.99.64)
No genes
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxybutanoyl-CoA + NAD+→acetoacetyl-CoA + NADH + H+ (EC 1.1.1.35)
PP_2047
PP_2136
PP_3755
Acetyl-CoA C-acyltransferase (in reverse):
acetoacetyl-CoA + coenzyme A→2 acetyl-CoA (EC 2.3.1.16; 2.3.1.9)
PP_0582
PP_1377
PP_2051
PP_2137
PP_2215
PP_3280
PP_3355
PP_3754
PP_4636

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information