MetaCyc Pathway: (8E,10E)-dodeca-8,10-dienol biosynthesis in Pseudomonas putida KT2440

Add experiment(s):


palmitoyl-CoA[in] + an oxidized electron-transfer flavoprotein[in] + H+[in]→(2E)-hexadec-2-enoyl-CoA[in] + a reduced electron-transfer flavoprotein[in] (EC 1.3.8.9)
No genes
(8E,10E)-dodeca-8,10-dienoate + 2 NADPH + 3 H+→(8E,10E)-dodeca-8,10-dienol + 2 NADP+ + H2O
No genes
Enoyl-CoA hydratase (in reverse):
(2E)-hexadec-2-enoyl-CoA + H2O→(S)-3-hydroxyhexadecanoyl-CoA (EC 4.2.1.17)
PP_1412
PP_1845
PP_2047
PP_2136
PP_2217
PP_3283
PP_3284
PP_3358
PP_3491
PP_3726
PP_3732
PP_4030
Long-chain-3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxyhexadecanoyl-CoA + NAD+→3-oxopalmitoyl-CoA + NADH + H+ (EC 1.1.1.211)
PP_2047
PP_2136
Acetyl-CoA C-myristoyltransferase (in reverse):
3-oxopalmitoyl-CoA + coenzyme A→myristoyl-CoA + acetyl-CoA (EC 2.3.1.155)
PP_3355
myristoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E)-tetradec-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.8)
No genes
Enoyl-CoA hydratase (in reverse):
(2E)-tetradec-2-enoyl-CoA + H2O→(S)-3-hydroxytetradecanoyl-CoA (EC 4.2.1.17)
PP_1412
PP_1845
PP_2047
PP_2136
PP_2217
PP_3283
PP_3284
PP_3358
PP_3491
PP_3726
PP_3732
PP_4030
(S)-3-hydroxytetradecanoyl-CoA + NAD+→3-oxomyristoyl-CoA + NADH + H+
PP_2047
PP_2136
Acetyl-CoA C-acyltransferase (in reverse):
3-oxomyristoyl-CoA + coenzyme A→lauroyl-CoA + acetyl-CoA (EC 2.3.1.16)
PP_0582
PP_1377
PP_2051
PP_2137
PP_2215
PP_3280
PP_3355
PP_3754
PP_4636
lauroyl-CoA + 2 a ferrocytochrome b5 + dioxygen + 2 H+→(9E)-dodecenoyl-CoA + 2 a ferricytochrome b5 + 2 H2O
No genes
(9E)-dodecenoyl-CoA + 2 a ferrocytochrome b5 + dioxygen + 2 H+→(8E,10E)-dodeca-8,10-dienoyl-CoA + 2 a ferricytochrome b5 + 2 H2O
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information