MetaCyc Pathway: isopropanol biosynthesis (engineered) in Pseudomonas putida KT2440

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Pyruvate synthase:
pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
No genes
Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
PP_0582
PP_1377
PP_2051
PP_2137
PP_2215
PP_3280
PP_3355
PP_3754
PP_4636
Acetoacetyl-CoA hydrolase:
acetoacetyl-CoA + H2O→acetoacetate + coenzyme A + H+ (EC 3.1.2.11)
No genes
Acetoacetate decarboxylase:
acetoacetate + H+→acetone + CO2 (EC 4.1.1.4)
No genes
Isopropanol dehydrogenase (NADP(+)) (in reverse):
acetone + NADPH + H+→propan-2-ol + NADP+ (EC 1.1.1.80)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information