MetaCyc Pathway: 3-methylbutanol biosynthesis (engineered) in Pseudomonas putida KT2440

MetaCyc Pathway: 3-methylbutanol biosynthesis (engineered) in Pseudomonas putida KT2440

2-isopropylmalate synthase:
3-methyl-2-oxobutanoate + acetyl-CoA + H₂O→(2S)-2-isopropylmalate + coenzyme A + H⁺ (EC 2.3.3.13)

3-isopropylmalate dehydratase (in reverse):
(2S)-2-isopropylmalate→2-isopropylmaleate + H₂O (EC 4.2.1.33)

3-isopropylmalate dehydratase (in reverse):
2-isopropylmaleate + H₂O→(2R,3S)-3-isopropylmalate (EC 4.2.1.33)

3-isopropylmalate dehydrogenase:
(2R,3S)-3-isopropylmalate + NAD⁺→(2S)-2-isopropyl-3-oxosuccinate + NADH + H⁺ (EC 1.1.1.85)

3-isopropylmalate dehydrogenase:
(2S)-2-isopropyl-3-oxosuccinate + H⁺→4-methyl-2-oxopentanoate + CO₂ (spontaneous) (EC 1.1.1.85)

Branched-chain-2-oxoacid decarboxylase:
4-methyl-2-oxopentanoate + H⁺→3-methylbutanal + CO₂ (EC 4.1.1.72)

No genes

Alcohol dehydrogenase (in reverse):
3-methylbutanal + NADH + H⁺→3-methylbutanol + NAD⁺ (EC 1.1.1.1)

Links:

Pathway details at MetaCyc
Fitness data for 11 genes
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All MetaCyc pathways for Pseudomonas putida KT2440

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information