MetaCyc Pathway: cis-vaccenate biosynthesis in Pseudomonas putida KT2440

Beta-ketoacyl-acyl-carrier-protein synthase II: a palmitoleoyl-[acp] + a malonyl-[acp] + H+→an (11Z)-3-oxooctadec-11-enoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)

PP_1916

PP_2778

PP_2780

PP_2781

PP_3303

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse): an (11Z)-3-oxooctadec-11-enoyl-[acp] + NADPH + H+→a (3R,11Z)-3-hydroxyoctadec-11-enoyl-[acp] + NADP+ (EC 1.1.1.100)

PP_0581

PP_1852

PP_1914

PP_1953

PP_2115

PP_2175

PP_2219

PP_2503

PP_2540

PP_2783

PP_3547

PP_4317

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase: a (3R,11Z)-3-hydroxyoctadec-11-enoyl-[acp]→a (2E,11Z)-octadeca-2,11-dienoyl-[acp] + H2O (EC 4.2.1.59)

PP_1602

PP_4174

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse): a (2E,11Z)-octadeca-2,11-dienoyl-[acp] + NADH + H+→a cis-vaccenoyl-[acp] + NAD+ (EC 1.3.1.9)

PP_3827

Oleoyl-[acyl-carrier-protein] hydrolase: a cis-vaccenoyl-[acp] + H2O→cis-vaccenate + a soluble [acyl-carrier protein] + H+ (EC 3.1.2.14)

PP_2318

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information