MetaCyc Pathway: L-citrulline biosynthesis in Pseudomonas putida KT2440

MetaCyc Pathway: L-citrulline biosynthesis in Pseudomonas putida KT2440

Arginase:
L-arginine + H₂O→L-ornithine + urea (EC 3.5.3.1)

No genes

Glutamate synthase (NADPH):
L-glutamine + H₂O→L-glutamate + ammonia + H⁺ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)

L-proline_[in] + an electron-transfer quinone→(S)-1-pyrroline-5-carboxylate_[in] + an electron-transfer quinol + H⁺_[in] (EC 1.5.5.2)

Glutamate 5-kinase:
L-glutamate + ATP→γ-L-glutamyl 5-phosphate + ADP (EC 2.7.2.11)

(S)-1-pyrroline-5-carboxylate + H⁺ + H₂O→L-glutamate 5-semialdehyde (spontaneous)

No genes

Glutamate-5-semialdehyde dehydrogenase (in reverse):
γ-L-glutamyl 5-phosphate + NADPH + H⁺→L-glutamate 5-semialdehyde + NADP⁺ + phosphate (EC 1.2.1.41)

Ornithine aminotransferase (in reverse):
L-glutamate + L-glutamate 5-semialdehyde→L-ornithine + 2-oxoglutarate (EC 2.6.1.13)

Ornithine carbamoyltransferase:
L-ornithine + carbamoyl phosphate→L-citrulline + H⁺ + phosphate (EC 2.1.3.3)

Links:

Pathway details at MetaCyc
Fitness data for 35 genes
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All MetaCyc pathways for Pseudomonas putida KT2440

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information