MetaCyc Pathway: L-asparagine biosynthesis III (tRNA-dependent) in Pseudomonas sp. S08-1

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Glutamate synthase (NADPH):
L-glutamine + H2O→ammonia + L-glutamate + H+ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)
OH686_01715
OH686_01765
OH686_04135
OH686_06395
OH686_08620
OH686_08625
OH686_08630
OH686_10000
OH686_10005
OH686_13005
OH686_13270
OH686_15125
OH686_15130
OH686_15680
OH686_17075
OH686_17080
OH686_17595
OH686_17715
OH686_18740
OH686_20460
OH686_20985
OH686_21970
OH686_22370
Aspartate--tRNA(Asn) ligase:
L-aspartate + tRNAAsn + ATP→an L-aspartyl-[tRNAAsn] + AMP + diphosphate (EC 6.1.1.23)
OH686_19115
Asparaginyl-tRNA synthase (glutamine-hydrolyzing):
an L-aspartyl-[tRNAAsn] + ATP→a 4-phosphooxy-L-aspartyl-[tRNAAsn] + ADP (EC 6.3.5.6)
OH686_04135
OH686_08620
OH686_08625
OH686_08630
Asparaginyl-tRNA synthase (glutamine-hydrolyzing):
a 4-phosphooxy-L-aspartyl-[tRNAAsn] + ammonia→an L-asparaginyl-[tRNAAsn] + phosphate (EC 6.3.5.6)
OH686_04135
OH686_08620
OH686_08625
OH686_08630

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information