MetaCyc Pathway: NiFe(CO)(CN)2 cofactor biosynthesis in Pseudomonas sp. S08-1

Add experiment(s):


[HypC dimer]-Fe-CO2 + a HypD [NiFe]-hydrogenase maturation protein→a [HypC/HypD dimer]-Fe-CO2 + a HypC [NiFe]-hydrogenase maturation protein
No genes
Ni2+ + GTP + a HypB hydrogenase accessory protein + H2O→Ni-[HypB hydrogenase accessory protein] + GDP + H+ + phosphate
OH686_06255
carbamoyl phosphate + ATP + a HypE [NiFe]-hydrogenase maturation protein + H2O→a [HypE hydrogenase maturation protein]-S-carbamoyl-L-cysteine + AMP + diphosphate + H+ + phosphate
No genes
a [HypE hydrogenase maturation protein]-S-carbamoyl-L-cysteine + ATP→a [HypE hydrogenase maturation protein]-S-cyanyl-L-cysteine + ADP + H+ + phosphate (EC 4.2.1.M8)
No genes
a [HypC/HypD dimer]-Fe-CO2 + ATP + 2 a reduced one electron carrier→[HypC/HypD dimer]-Fe-CO + ADP + 2 an oxidized electron carrier + H+ + phosphate
No genes
Ni-[HypB hydrogenase accessory protein] + a HypA hydrogenase accessory protein→Ni-[HypA hydrogenase accessory protein] + a HypB hydrogenase accessory protein
No genes
[HypC/HypD dimer]-Fe-CO + 2 a [HypE hydrogenase maturation protein]-S-cyanyl-L-cysteine→[HypCDE]-Fe(CO)(CN)2
No genes
[HypCDE]-Fe(CO)(CN)2 + a [NiFe]-hydrogenase large subunit→{[NiFe]-hydrogenase large subunit}-Fe(CO)(CN)2 + a HypCDE trimer
No genes
{[NiFe]-hydrogenase large subunit}-Fe(CO)(CN)2 + Ni-[HypA hydrogenase accessory protein]→[NiFe]-hydrogenase large subunit with unformed NiFe(CO)(CN)2 cofactor + a HypA hydrogenase accessory protein
No genes
[NiFe]-hydrogenase large subunit with unformed NiFe(CO)(CN)2 cofactor + H2O→holo-[NiFe]-hydrogenase large subunit + an oligopeptide (EC 3.4.23.51)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information