MetaCyc Pathway: toluene degradation VI (anaerobic) in Pseudomonas sp. S08-1

Add experiment(s):


Benzylsuccinate synthase (in reverse):
toluene + fumarate→(R)-2-benzylsuccinate (EC 4.1.99.11)
No genes
Succinyl-CoA:(R)-benzylsuccinate CoA-transferase:
(R)-2-benzylsuccinate + succinyl-CoA→(R)-benzylsuccinyl-CoA + succinate (EC 2.8.3.15)
No genes
(R)-benzylsuccinyl-CoA + an oxidized electron-transfer flavoprotein + H+→(E)-2-benzylidenesuccinyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.3)
No genes
(E)-2-benzylidenesuccinyl-CoA + H2O→(2S)-2-[(R)-α-hydroxybenzyl]succinyl-CoA (EC 4.2.1.180)
No genes
(2S)-2-[(R)-α-hydroxybenzyl]succinyl-CoA + NAD+→(S)-2-benzoylsuccinyl-CoA + NADH + H+ (EC 1.1.1.429)
No genes
(S)-2-benzoylsuccinyl-CoA + coenzyme A→benzoyl-CoA + succinyl-CoA (EC 2.3.1.310)
No genes
benzoyl-CoA + 2 ATP + 2 a reduced ferredoxin [iron-sulfur] cluster + 2 H2O→cyclohex-1,5-diene-1-carbonyl-CoA + 2 ADP + 2 an oxidized ferredoxin [iron-sulfur] cluster + 2 phosphate (EC 1.3.7.8)
No genes
Cyclohexa-1,5-dienecarbonyl-CoA hydratase (in reverse):
cyclohex-1,5-diene-1-carbonyl-CoA + H2O→6-hydroxycyclohex-1-ene-1-carbonyl-CoA (EC 4.2.1.100)
No genes
6-hydroxycyclohex-1-ene-1-carbonyl-CoA + NAD+→6-oxocyclohex-1-ene-1-carbonyl-CoA + NADH + H+ (EC 1.1.1.368)
No genes
6-oxocyclohex-1-ene-1-carbonyl-CoA + H2O→2-hydroxy-6-oxocyclohexane-1-carbonyl-CoA (EC 3.7.1.21)
No genes
2-hydroxy-6-oxocyclohexane-1-carbonyl-CoA + H2O→3-hydroxypimeloyl-CoA + H+ (EC 3.7.1.21)
No genes
3-hydroxypimeloyl-CoA dehydrogenase:
3-hydroxypimeloyl-CoA + NAD+→3-oxopimeloyl-CoA + NADH + H+ (EC 1.1.1.259)
No genes
3-oxopimeloyl-CoA + coenzyme A→glutaryl-CoA + acetyl-CoA
OH686_17910
glutaryl-CoA + an oxidized electron-transfer flavoprotein + H+→(E)-glutaconyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.6)
No genes
(E)-glutaconyl-CoA + H+→crotonyl-CoA + CO2 (EC 1.3.8.6)
No genes
crotonyl-CoA + H2O→(S)-3-hydroxybutanoyl-CoA (EC 4.2.1.150)
OH686_09110
OH686_17935
OH686_18725
OH686_18795
OH686_21925
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxybutanoyl-CoA + NAD+→acetoacetyl-CoA + NADH + H+ (EC 1.1.1.35)
OH686_00865
OH686_05875
OH686_17935
OH686_21925
OH686_22350
Acetyl-CoA C-acyltransferase (in reverse):
acetoacetyl-CoA + coenzyme A→2 acetyl-CoA (EC 2.3.1.16; 2.3.1.9)
OH686_00870
OH686_06330
OH686_17215
OH686_17910
OH686_21930
OH686_22345
OH686_23885

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information