MetaCyc Pathway: valproate β-oxidation in Pseudomonas sp. RS175

MetaCyc Pathway: valproate β-oxidation in Pseudomonas sp. RS175

Butyrate--CoA ligase:
valproate + ATP + coenzyme A→valproyl-CoA + AMP + diphosphate (EC 6.2.1.2)

No genes

valproyl-CoA + an oxidized electron-transfer flavoprotein + H⁺→(2E)-2-propylpent-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)

No genes

Enoyl-CoA hydratase (in reverse):
(2E)-2-propylpent-2-enoyl-CoA + H₂O→(3S)-3-hydroxy-2-propylpentanoyl-CoA (EC 4.2.1.17)

3-hydroxyacyl-CoA dehydrogenase:
(3S)-3-hydroxy-2-propylpentanoyl-CoA + NAD⁺→3-oxo-2-propylpentanoyl-CoA + NADH + H⁺ (EC 1.1.1.35)

Acetyl-CoA C-acyltransferase (in reverse):
3-oxo-2-propylpentanoyl-CoA + coenzyme A→pentanoyl-CoA + propanoyl-CoA (EC 2.3.1.16)

pentanoyl-CoA + an oxidized electron-transfer flavoprotein + H⁺→(2E)-pent-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.1)

(2E)-pent-2-enoyl-CoA + H₂O→(3S)-3-hydroxypentanoyl-CoA

No genes

3-hydroxyacyl-CoA dehydrogenase:
(3S)-3-hydroxypentanoyl-CoA + NAD⁺→3-oxopentanoyl-CoA + NADH + H⁺ (EC 1.1.1.35)

Acetyl-CoA C-acyltransferase (in reverse):
3-oxopentanoyl-CoA + coenzyme A→propanoyl-CoA + acetyl-CoA (EC 2.3.1.16; 2.3.1.9)

Links:

Pathway details at MetaCyc
Fitness data for 20 genes
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All MetaCyc pathways for Pseudomonas sp. RS175

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information