MetaCyc Pathway: (8<i>E</i>,10<i>E</i>)-dodeca-8,10-dienol biosynthesis in Pseudomonas sp. RS175

MetaCyc Pathway: (8E,10E)-dodeca-8,10-dienol biosynthesis in Pseudomonas sp. RS175

palmitoyl-CoA_[in] + an oxidized electron-transfer flavoprotein_[in] + H⁺_[in]→(2E)-hexadec-2-enoyl-CoA_[in] + a reduced electron-transfer flavoprotein_[in] (EC 1.3.8.9)

No genes

(8E,10E)-dodeca-8,10-dienoate + 2 NADPH + 3 H⁺→(8E,10E)-dodeca-8,10-dienol + 2 NADP⁺ + H₂O

No genes

Enoyl-CoA hydratase (in reverse):
(2E)-hexadec-2-enoyl-CoA + H₂O→(S)-3-hydroxyhexadecanoyl-CoA (EC 4.2.1.17)

Long-chain-3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxyhexadecanoyl-CoA + NAD⁺→3-oxopalmitoyl-CoA + NADH + H⁺ (EC 1.1.1.211)

Acetyl-CoA C-myristoyltransferase (in reverse):
3-oxopalmitoyl-CoA + coenzyme A→myristoyl-CoA + acetyl-CoA (EC 2.3.1.155)

myristoyl-CoA + an oxidized electron-transfer flavoprotein + H⁺→(2E)-tetradec-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.8)

No genes

Enoyl-CoA hydratase (in reverse):
(2E)-tetradec-2-enoyl-CoA + H₂O→(S)-3-hydroxytetradecanoyl-CoA (EC 4.2.1.17)

(S)-3-hydroxytetradecanoyl-CoA + NAD⁺→3-oxomyristoyl-CoA + NADH + H⁺

Acetyl-CoA C-acyltransferase (in reverse):
3-oxomyristoyl-CoA + coenzyme A→lauroyl-CoA + acetyl-CoA (EC 2.3.1.16)

lauroyl-CoA + 2 a ferrocytochrome b₅ + dioxygen + 2 H⁺→(9E)-dodecenoyl-CoA + 2 a ferricytochrome b₅ + 2 H₂O

No genes

(9E)-dodecenoyl-CoA + 2 a ferrocytochrome b₅ + dioxygen + 2 H⁺→(8E,10E)-dodeca-8,10-dienoyl-CoA + 2 a ferricytochrome b₅ + 2 H₂O

No genes

Links:

Pathway details at MetaCyc
Fitness data for 18 genes
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All MetaCyc pathways for Pseudomonas sp. RS175

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information