MetaCyc Pathway: adipate degradation in Pseudomonas fluorescens SBW25

MetaCyc Pathway: adipate degradation in Pseudomonas fluorescens SBW25

Succinate--CoA ligase (ADP-forming):
adipate + ATP + coenzyme A→adipoyl-CoA + ADP + phosphate (EC 6.2.1.5)

adipoyl-CoA + an oxidized electron-transfer flavoprotein + H⁺→(2E)-5-carboxy-2-pentenoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.7)

Enoyl-CoA hydratase (in reverse):
(2E)-5-carboxy-2-pentenoyl-CoA + H₂O→(3S)-hydroxyadipyl-CoA (EC 4.2.1.17)

3-hydroxyacyl-CoA dehydrogenase:
(3S)-hydroxyadipyl-CoA + NAD⁺→3-oxoadipoyl-CoA + NADH + H⁺ (EC 1.1.1.35)

3-oxoadipyl-CoA thiolase (in reverse):
3-oxoadipoyl-CoA + coenzyme A→succinyl-CoA + acetyl-CoA (EC 2.3.1.174)

Links:

Pathway details at MetaCyc
Fitness data for 22 genes
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All MetaCyc pathways for Pseudomonas fluorescens SBW25

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information