MetaCyc Pathway: C4 photosynthetic carbon assimilation cycle, PEPCK type in Pseudomonas fluorescens SBW25

MetaCyc Pathway: C4 photosynthetic carbon assimilation cycle, PEPCK type in Pseudomonas fluorescens SBW25

Carbonate dehydratase (in reverse):
CO₂ + H₂O→hydrogen carbonate + H⁺ (EC 4.2.1.1)

Phosphoenolpyruvate carboxylase (in reverse):
hydrogen carbonate + phosphoenolpyruvate→oxaloacetate + phosphate (EC 4.1.1.31)

Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)

Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)) (in reverse):
oxaloacetate + NADPH + H⁺→(S)-malate + NADP⁺ (EC 1.1.1.40; 1.1.1.82)

L-aspartate_[in]→L-aspartate_[out]

No genes

(S)-malate_[in]→(S)-malate_[out]

No genes

Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)):
(S)-malate + NADP⁺→CO₂ + pyruvate + NADPH (EC 1.1.1.40)

Aspartate transaminase:
L-aspartate + 2-oxoglutarate→oxaloacetate + L-glutamate (EC 2.6.1.1)

Alanine transaminase (in reverse):
pyruvate + L-glutamate→L-alanine + 2-oxoglutarate (EC 2.6.1.2)

Phosphoenolpyruvate carboxykinase (ATP):
oxaloacetate + ATP→CO₂ + phosphoenolpyruvate + ADP (EC 4.1.1.49)

L-alanine_[in]→L-alanine_[out]

No genes

phosphoenolpyruvate_[in]→phosphoenolpyruvate_[out]

No genes

Alanine transaminase (in reverse):
L-alanine + 2-oxoglutarate→pyruvate + L-glutamate (EC 2.6.1.2)

Pyruvate, phosphate dikinase:
pyruvate + ATP + phosphate→phosphoenolpyruvate + AMP + diphosphate + H⁺ (EC 2.7.9.1)

No genes

Links:

Pathway details at MetaCyc
Fitness data for 24 genes
Select organism:
All MetaCyc pathways for Pseudomonas fluorescens SBW25

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information