MetaCyc Pathway: C4 photosynthetic carbon assimilation cycle, NAD-ME type in Pseudomonas fluorescens SBW25

MetaCyc Pathway: C4 photosynthetic carbon assimilation cycle, NAD-ME type in Pseudomonas fluorescens SBW25

Carbonate dehydratase (in reverse):
CO₂ + H₂O→hydrogen carbonate + H⁺ (EC 4.2.1.1)

Phosphoenolpyruvate carboxylase (in reverse):
hydrogen carbonate + phosphoenolpyruvate→oxaloacetate + phosphate (EC 4.1.1.31)

Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)

L-aspartate_[in]→L-aspartate_[out]

No genes

Aspartate transaminase:
L-aspartate + 2-oxoglutarate→oxaloacetate + L-glutamate (EC 2.6.1.1)

Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H⁺→(S)-malate + NAD⁺ (EC 1.1.1.37; 1.1.1.38)

No genes

Malate dehydrogenase (oxaloacetate-decarboxylating):
(S)-malate + NAD⁺→CO₂ + pyruvate + NADH (EC 1.1.1.38)

No genes

Alanine transaminase (in reverse):
pyruvate + L-glutamate→L-alanine + 2-oxoglutarate (EC 2.6.1.2)

L-alanine_[in]→L-alanine_[out]

No genes

Alanine transaminase (in reverse):
L-alanine + 2-oxoglutarate→pyruvate + L-glutamate (EC 2.6.1.2)

Pyruvate, phosphate dikinase:
pyruvate + ATP + phosphate→phosphoenolpyruvate + AMP + diphosphate + H⁺ (EC 2.7.9.1)

No genes

Links:

Pathway details at MetaCyc
Fitness data for 22 genes
Select organism:
All MetaCyc pathways for Pseudomonas fluorescens SBW25

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information