MetaCyc Pathway: hexitol fermentation to lactate, formate, ethanol and acetate in Pseudomonas fluorescens SBW25

Add experiment(s):


Mannitol-1-phosphate 5-dehydrogenase:
D-mannitol 1-phosphate + NAD+→β-D-fructofuranose 6-phosphate + NADH + H+ (EC 1.1.1.17)
No genes
Sorbitol-6-phosphate 2-dehydrogenase:
D-sorbitol 6-phosphate + NAD+→keto-D-fructose 6-phosphate + NADH + H+ (EC 1.1.1.140)
PFLU_RS15455
6-phosphofructokinase:
β-D-fructofuranose 6-phosphate + ATP→β-D-fructofuranose 1,6-bisphosphate + ADP + H+ (EC 2.7.1.11)
No genes
Fructose-bisphosphate aldolase:
β-D-fructofuranose 1,6-bisphosphate→glycerone phosphate + D-glyceraldehyde 3-phosphate (EC 4.1.2.13)
PFLU_RS28070
Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H2O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)
PFLU_RS01745
Triose-phosphate isomerase (in reverse):
glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)
PFLU_RS25840
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
PFLU_RS07715
PFLU_RS24335
Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
PFLU_RS28085
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
PFLU_RS01655
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.11)
No genes
Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
PFLU_RS06375
PFLU_RS09395
Pyruvate, water dikinase:
pyruvate + ATP + H2O→phosphoenolpyruvate + AMP + 2 H+ + phosphate (EC 2.7.9.2)
PFLU_RS22630
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
PFLU_RS08850
PFLU_RS24255
L-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(S)-lactate + NAD+ (EC 1.1.1.27)
No genes
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
No genes
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
No genes
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
PFLU_RS03780
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
PFLU_RS24605
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
PFLU_RS06395
PFLU_RS06965
PFLU_RS10840
PFLU_RS11825
PFLU_RS20115
PFLU_RS22935
PFLU_RS27395

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information