MetaCyc Pathway: superpathway of L-lysine, L-threonine and L-methionine biosynthesis I in Pseudomonas fluorescens SBW25

Add experiment(s):


Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate
(EC 2.6.1.1)
PFLU_RS08510
PFLU_RS09125
PFLU_RS10980
PFLU_RS13455
PFLU_RS15480
PFLU_RS16880
PFLU_RS17055
PFLU_RS17810
PFLU_RS19680
PFLU_RS21080
PFLU_RS21355
PFLU_RS21880
PFLU_RS22640
PFLU_RS22895
PFLU_RS24775
PFLU_RS29405
PFLU_RS29850
Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP
(EC 2.7.2.4)
PFLU_RS23265
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate
(EC 1.2.1.11)
PFLU_RS06680
PFLU_RS20555
PFLU_RS20560
L-aspartate 4-semialdehyde + pyruvate→(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H+ + H2O (EC 4.3.3.7)
PFLU_RS06945
PFLU_RS07275
PFLU_RS09565
PFLU_RS16615
Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+
(EC 1.1.1.3)
PFLU_RS09890
PFLU_RS24550
Homoserine kinase:
L-homoserine + ATP→O-phospho-L-homoserine + ADP + H+
(EC 2.7.1.39)
PFLU_RS00385
PFLU_RS22735
Homoserine O-succinyltransferase:
L-homoserine + succinyl-CoA→O-succinyl-L-homoserine + coenzyme A
(EC 2.3.1.46)
No genes
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H + H+→(S)-2,3,4,5-tetrahydrodipicolinate + NAD(P)+ + H2O (EC 1.17.1.8)
PFLU_RS25890
Cystathionine gamma-synthase:
O-succinyl-L-homoserine + L-cysteine→L-cystathionine + H+ + succinate
(EC 2.5.1.48)
PFLU_RS02250
PFLU_RS16625
PFLU_RS20510
2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase:
(S)-2,3,4,5-tetrahydrodipicolinate + succinyl-CoA + H2ON-succinyl-2-amino-6-ketopimelate + coenzyme A
(EC 2.3.1.117)
PFLU_RS06245
Threonine synthase:
O-phospho-L-homoserine + H2O→L-threonine + phosphate
(EC 4.2.3.1)
PFLU_RS09885
PFLU_RS24545
Cysteine-S-conjugate beta-lyase:
L-cystathionine + H2O→L-homocysteine + ammonium + pyruvate
(EC 4.4.1.13)
No genes
Succinyldiaminopimelate transaminase (in reverse):
N-succinyl-2-amino-6-ketopimelate + L-glutamateN-succinyl-L,L-2,6-diaminopimelate + 2-oxoglutarate
(EC 2.6.1.17)
PFLU_RS06260
PFLU_RS23315
PFLU_RS27595
5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase:
L-homocysteine + 5-methyltetrahydropteroyl tri-L-glutamate→L-methionine + tetrahydropteroyl tri-L-glutamate
(EC 2.1.1.14)
PFLU_RS17405
Methionine synthase:
L-homocysteine + a 5-methyltetrahydrofolate→L-methionine + a tetrahydrofolate
(EC 2.1.1.13)
PFLU_RS13000
Succinyl-diaminopimelate desuccinylase:
N-succinyl-L,L-2,6-diaminopimelate + H2O→L,L-diaminopimelate + succinate
(EC 3.5.1.18)
PFLU_RS06220
Diaminopimelate epimerase:
L,L-diaminopimelate→meso-diaminopimelate
(EC 5.1.1.7)
PFLU_RS10355
PFLU_RS13140
PFLU_RS17335
PFLU_RS23725
PFLU_RS29270
Diaminopimelate decarboxylase:
meso-diaminopimelate + H+→L-lysine + CO2
(EC 4.1.1.20)
PFLU_RS29265

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information