MetaCyc Pathway: colanic acid building blocks biosynthesis in Pseudomonas fluorescens SBW25

MetaCyc Pathway: colanic acid building blocks biosynthesis in Pseudomonas fluorescens SBW25

Glucose-6-phosphate isomerase:
α-D-glucose 6-phosphate→β-D-fructofuranose 6-phosphate (EC 5.3.1.9)

Phosphoglucomutase (in reverse):
D-glucopyranose 6-phosphate→α-D-glucopyranose 1-phosphate (EC 5.4.2.2)

UTP-monosaccharide-1-phosphate uridylyltransferase:
α-D-glucopyranose 1-phosphate + H⁺ + UTP→UDP-α-D-glucose + diphosphate (EC 2.7.7.64; 2.7.7.9)

Mannose-6-phosphate isomerase (in reverse):
β-D-fructofuranose 6-phosphate→D-mannopyranose 6-phosphate (EC 5.3.1.8)

Phosphomannomutase (in reverse):
D-mannopyranose 6-phosphate→α-D-mannose 1-phosphate (EC 5.4.2.8)

UDP-glucose 4-epimerase:
UDP-α-D-glucose→UDP-α-D-galactose (EC 5.1.3.2)

UDP-glucose 6-dehydrogenase:
UDP-α-D-glucose + 2 NAD⁺ + H₂O→UDP-α-D-glucuronate + 2 NADH + 3 H⁺ (EC 1.1.1.22)

Mannose-1-phosphate guanylyltransferase:
α-D-mannose 1-phosphate + GTP + H⁺→GDP-α-D-mannose + diphosphate (EC 2.7.7.13)

GDP-mannose 4,6-dehydratase:
GDP-α-D-mannose→GDP-4-dehydro-α-D-rhamnose + H₂O (EC 4.2.1.47)

GDP-L-fucose synthase:
GDP-4-dehydro-α-D-rhamnose→GDP-4-dehydro-6-deoxy-β-L-galactose (EC 1.1.1.271)

GDP-L-fucose synthase (in reverse):
GDP-4-dehydro-6-deoxy-β-L-galactose + NADPH + H⁺→GDP-β-L-fucose + NADP⁺ (EC 1.1.1.271)

Links:

Pathway details at MetaCyc
Fitness data for 20 genes
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All MetaCyc pathways for Pseudomonas fluorescens SBW25

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information