MetaCyc Pathway: indole-3-acetate biosynthesis II in Paraburkholderia sabiae LMG 24235

Add experiment(s):


L-tryptophan + H+→tryptamine + CO2 (EC 4.1.1.105; 4.1.1.28)
QEN71_RS32485
QEN71_RS33975
L-tryptophan + dioxygen + a reduced [NADPH-hemoprotein reductase]→N2-hydroxy-L-tryptophan + an oxidized [NADPH-hemoprotein reductase] + H+ + H2O (EC 1.14.14.156)
No genes
Aspartate transaminase:
L-tryptophan + 2-oxoglutarate→(indol-3-yl)pyruvate + L-glutamate (EC 2.6.1.1; 2.6.1.27)
QEN71_RS00075
QEN71_RS02890
QEN71_RS03175
QEN71_RS03275
QEN71_RS06560
QEN71_RS08880
QEN71_RS10500
QEN71_RS12205
QEN71_RS12215
QEN71_RS13030
QEN71_RS17025
QEN71_RS18160
QEN71_RS18375
QEN71_RS20720
QEN71_RS21505
QEN71_RS22410
QEN71_RS23125
QEN71_RS23470
QEN71_RS24010
QEN71_RS24645
QEN71_RS30710
QEN71_RS31640
QEN71_RS32300
QEN71_RS36905
QEN71_RS38860
QEN71_RS38900
QEN71_RS38970
N2-hydroxy-L-tryptophan + dioxygen + a reduced [NADPH-hemoprotein reductase]→N2,N2-dihydroxy-L-tryptophan + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.156)
No genes
tryptamine + NADPH + dioxygen→N-hydroxyl-tryptamine + NADP+ + H2O
No genes
(indol-3-yl)pyruvate + NADPH + dioxygen + H+→(indol-3-yl)acetate + CO2 + NADP+ + H2O (EC 1.14.13.168)
No genes
N2,N2-dihydroxy-L-tryptophan + H+→(E)-(indol-3-yl)acetaldehyde oxime + CO2 + H2O (spontaneous) (EC 1.14.14.156)
No genes
N-hydroxyl-tryptamine + an oxidized electron carrier→(E)-(indol-3-yl)acetaldehyde oxime + a reduced two electron carrier
No genes
(E)-(indol-3-yl)acetaldehyde oxime→(indol-3-yl)acetonitrile + H2O (EC 4.8.1.3)
No genes
Nitrilase:
(indol-3-yl)acetonitrile + 2 H2O→(indol-3-yl)acetate + ammonium (EC 3.5.5.1)
QEN71_RS21435
QEN71_RS26550
Nitrile hydratase (in reverse):
(indol-3-yl)acetonitrile + H2O→(indol-3-yl)acetamide (EC 4.2.1.84)
QEN71_RS20580
QEN71_RS20585
Amidase:
(indol-3-yl)acetamide + H2O→(indol-3-yl)acetate + ammonium (EC 3.5.1.4)
QEN71_RS00590
QEN71_RS06970
QEN71_RS07500
QEN71_RS12695
QEN71_RS21055
QEN71_RS34425
QEN71_RS42445

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information