MetaCyc Pathway: formaldehyde assimilation I (serine pathway) in Paraburkholderia sabiae LMG 24235

MetaCyc Pathway: formaldehyde assimilation I (serine pathway) in Paraburkholderia sabiae LMG 24235

formaldehyde + a tetrahydrofolate→a 5,10-methylenetetrahydrofolate + H₂O (spontaneous)

No genes

Glycine hydroxymethyltransferase (in reverse):
a 5,10-methylenetetrahydrofolate + glycine + H₂O→L-serine + a tetrahydrofolate (EC 2.1.2.1)

Serine--glyoxylate transaminase:
L-serine + glyoxylate→glycine + 3-hydroxypyruvate (EC 2.6.1.45)

Hydroxypyruvate reductase (in reverse):
3-hydroxypyruvate + NAD(P)H + H⁺→D-glycerate + NAD(P)⁺ (EC 1.1.1.81)

D-glycerate + ATP→2-phospho-D-glycerate + ADP + H⁺ (EC 2.7.1.165)

Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H₂O (EC 4.2.1.11)

2-phospho-D-glycerate→3-phospho-D-glycerate (EC 5.4.2.11)

Phosphoenolpyruvate carboxylase (in reverse):
phosphoenolpyruvate + hydrogen carbonate→oxaloacetate + phosphate (EC 4.1.1.31)

Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H⁺→(S)-malate + NAD⁺ (EC 1.1.1.37; 1.1.1.38)

Malate--CoA ligase:
(S)-malate + ATP + coenzyme A→(S)-malyl-CoA + ADP + phosphate (EC 6.2.1.9)

Malyl-CoA lyase:
(S)-malyl-CoA→glyoxylate + acetyl-CoA (EC 4.1.3.24)

2 acetyl-CoA + CO₂ + an oxidized electron-transfer flavoprotein + 2 NADPH + 2 H⁺→glyoxylate + coenzyme A + a reduced electron-transfer flavoprotein + 2 NADP⁺ + propanoyl-CoA

No genes

Serine--glyoxylate transaminase (in reverse):
glyoxylate + L-serine→glycine + 3-hydroxypyruvate (EC 2.6.1.45)

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information