MetaCyc Pathway: 5,6-dehydrokavain biosynthesis (engineered) in Hydrogenophaga sp. GW460-11-11-14-LB1

MetaCyc Pathway: 5,6-dehydrokavain biosynthesis (engineered) in Hydrogenophaga sp. GW460-11-11-14-LB1

3-phenylpropanoate + ATP + coenzyme A→3-phenylpropanoyl-CoA + AMP + diphosphate

No genes

trans-cinnamate + ATP + coenzyme A→(E)-cinnamoyl-CoA + AMP + diphosphate

No genes

Acetyl-CoA C-acyltransferase:
(E)-cinnamoyl-CoA + acetyl-CoA→(4E)-3-oxo-5-phenylpent-4-enoyl-CoA + coenzyme A (EC 2.3.1.16)

Acetyl-CoA C-acyltransferase:
3-phenylpropanoyl-CoA + acetyl-CoA→3-oxo-5-phenylpentanoyl-CoA + coenzyme A (EC 2.3.1.16)

Acetyl-CoA C-acyltransferase:
(4E)-3-oxo-5-phenylpent-4-enoyl-CoA + acetyl-CoA→(6E)-3,5-dioxo-7-phenylhept-6-enoyl-CoA + coenzyme A (EC 2.3.1.16)

Acetyl-CoA C-acyltransferase:
3-oxo-5-phenylpentanoyl-CoA + acetyl-CoA→3,5-dioxo-7-phenylheptanoyl-CoA + coenzyme A (EC 2.3.1.16)

(6E)-3,5-dioxo-7-phenylhept-6-enoyl-CoA→(E)-4-hydroxy-6-(styryl)pyran-2-one + coenzyme A (spontaneous)

No genes

3,5-dioxo-7-phenylheptanoyl-CoA→4-hydroxy-6-(penethyl)pyran-2-one + coenzyme A (spontaneous)

No genes

(E)-4-hydroxy-6-(styryl)pyran-2-one + S-adenosyl-L-methionine→5,6-dehydrokavain + S-adenosyl-L-homocysteine + H⁺ (EC 2.1.1.M66)

No genes

4-hydroxy-6-(penethyl)pyran-2-one + S-adenosyl-L-methionine→dihydro-5,6-dehydrokavain + S-adenosyl-L-homocysteine + H⁺ (EC 2.1.1.M66)

No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information