MetaCyc Pathway: (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) in Hydrogenophaga sp. GW460-11-11-14-LB1

Add experiment(s):


Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
GFF1723
GFF2108
GFF3471
GFF3554
GFF3722
GFF3782
GFF3813
GFF415
GFF429
GFF437
GFF4593
GFF4796
GFF4825
GFF5621
GFF5633
GFF5665
GFF5843
GFF678
GFF876
GFF906
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
GFF2109
GFF376
GFF3825
GFF414
GFF4497
GFF4592
GFF4826
GFF5620
GFF5662
GFF875
Acetoacetyl-CoA reductase (in reverse):
acetoacetyl-CoA + NADPH + H+→(3R)-3-hydroxybutanoyl-CoA + NADP+ (EC 1.1.1.36)
GFF4795
(S)-3-hydroxybutanoyl-CoA + H2O→(S)-3-hydroxybutanoate + coenzyme A + H+
No genes
(3R)-3-hydroxybutanoyl-CoA + H2O→(R)-3-hydroxybutanoate + coenzyme A + H+
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information