MetaCyc Pathway: pyruvate fermentation to isobutanol (engineered) in Hydrogenophaga sp. GW460-11-11-14-LB1

Add experiment(s):


Acetolactate synthase:
2 pyruvate + H+→(S)-2-acetolactate + CO2 (EC 2.2.1.6)
GFF11
GFF12
GFF1551
GFF2148
GFF2981
GFF3744
GFF3745
GFF3965
GFF4276
GFF4277
GFF445
GFF5606
Ketol-acid reductoisomerase (in reverse):
(S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ (EC 1.1.1.86)
GFF4278
Dihydroxy-acid dehydratase:
(2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O (EC 4.2.1.9)
GFF2073
GFF2098
GFF4111
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
GFF2166
GFF3318
GFF3549
GFF4409
GFF4656
GFF5004
GFF5778
GFF5851

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information