MetaCyc Pathway: pyruvate fermentation to isobutanol (engineered) in Pectobacterium carotovorum WPP14

Add experiment(s):


Acetolactate synthase:
2 pyruvate + H+→(S)-2-acetolactate + CO2 (EC 2.2.1.6)
HER17_RS00335
HER17_RS01060
HER17_RS01065
HER17_RS03010
HER17_RS03015
HER17_RS03095
HER17_RS03100
HER17_RS18035
HER17_RS19205
Ketol-acid reductoisomerase (in reverse):
(S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ (EC 1.1.1.86)
HER17_RS01100
Dihydroxy-acid dehydratase:
(2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O (EC 4.2.1.9)
HER17_RS01075
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
HER17_RS02485
HER17_RS03445
HER17_RS06255
HER17_RS11030
HER17_RS12790
HER17_RS13680
HER17_RS17865

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information