MetaCyc Pathway: 3-methylbutanol biosynthesis (engineered) in Pantoea agglomerans CFBP13505 P0401

Add experiment(s):


2-isopropylmalate synthase:
3-methyl-2-oxobutanoate + acetyl-CoA + H2O→(2S)-2-isopropylmalate + coenzyme A + H+ (EC 2.3.3.13)
PagCFBP13516_RS10400 (leuA)
PagCFBP13516_RS16310 (leuA)
3-isopropylmalate dehydratase (in reverse):
(2S)-2-isopropylmalate→2-isopropylmaleate + H2O (EC 4.2.1.33)
PagCFBP13516_RS16320 (leuC)
PagCFBP13516_RS16325 (leuD)
3-isopropylmalate dehydratase (in reverse):
2-isopropylmaleate + H2O→(2R,3S)-3-isopropylmalate (EC 4.2.1.33)
PagCFBP13516_RS16320 (leuC)
PagCFBP13516_RS16325 (leuD)
3-isopropylmalate dehydrogenase:
(2R,3S)-3-isopropylmalate + NAD+→(2S)-2-isopropyl-3-oxosuccinate + NADH + H+ (EC 1.1.1.85)
PagCFBP13516_RS16315 (leuB)
3-isopropylmalate dehydrogenase:
(2S)-2-isopropyl-3-oxosuccinate + H+→4-methyl-2-oxopentanoate + CO2 (spontaneous) (EC 1.1.1.85)
PagCFBP13516_RS16315 (leuB)
Branched-chain-2-oxoacid decarboxylase:
4-methyl-2-oxopentanoate + H+→3-methylbutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
3-methylbutanal + NADH + H+→3-methylbutanol + NAD+ (EC 1.1.1.1)
PagCFBP13516_RS01140
PagCFBP13516_RS03775 (acuI)
PagCFBP13516_RS07075
PagCFBP13516_RS08740 (adhP)
PagCFBP13516_RS09190 (adhE)
PagCFBP13516_RS14230
PagCFBP13516_RS16050
PagCFBP13516_RS18055
PagCFBP13516_RS19800
PagCFBP13516_RS22100 (ahr)

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information