MetaCyc Pathway: superpathway of rosmarinic acid biosynthesis in Pantoea agglomerans CFBP13505 P0401

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Phenylalanine ammonia-lyase:
L-phenylalanine→trans-cinnamate + ammonium (EC 4.3.1.24; 4.3.1.25; 5.4.3.10)
No genes
Tyrosine 3-monooxygenase:
L-tyrosine + dioxygen + 5,6,7,8-tetrahydrobiopterin→L-dopa + a 4a-hydroxytetrahydrobiopterin (EC 1.14.16.2)
No genes
Aspartate transaminase:
L-tyrosine + 2-oxoglutarate→3-(4-hydroxyphenyl)pyruvate + L-glutamate (EC 2.6.1.1; 2.6.1.27; 2.6.1.5; 2.6.1.57)
PagCFBP13516_RS00080
PagCFBP13516_RS01090
PagCFBP13516_RS01115 (pdxR)
PagCFBP13516_RS03520
PagCFBP13516_RS03525
PagCFBP13516_RS06375 (pdxR)
PagCFBP13516_RS06560
PagCFBP13516_RS07470 (hisC)
PagCFBP13516_RS09780
PagCFBP13516_RS12850
PagCFBP13516_RS14200
PagCFBP13516_RS18070
PagCFBP13516_RS18340
PagCFBP13516_RS23715
Hydroxyphenylpyruvate reductase (in reverse):
3-(4-hydroxyphenyl)pyruvate + NAD(P)H + H+→(R)-3-(4-hydroxyphenyl)lactate + NAD(P)+ (EC 1.1.1.237)
PagCFBP13516_RS01245
Dihydroxyphenylalanine transaminase:
L-dopa + 2-oxoglutarate→3-(3,4-dihydroxyphenyl)pyruvate + L-glutamate (EC 2.6.1.49)
No genes
trans-cinnamate + dioxygen + a reduced [NADPH-hemoprotein reductase]→4-coumarate + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.91)
No genes
4-coumarate--CoA ligase:
4-coumarate + ATP + coenzyme A→(E)-4-coumaroyl-CoA + AMP + diphosphate (EC 6.2.1.12)
No genes
Hydroxyphenylpyruvate reductase (in reverse):
3-(3,4-dihydroxyphenyl)pyruvate + NAD(P)H + H+→(R)-3-(3,4-dihydroxyphenyl)lactate + NAD(P)+ (EC 1.1.1.237)
PagCFBP13516_RS01245
(R)-3-(4-hydroxyphenyl)lactate + (E)-4-coumaroyl-CoA→4-coumaroyl-4'-hydroxyphenyllactate + coenzyme A
No genes
Rosmarinate synthase:
(R)-3-(3,4-dihydroxyphenyl)lactate + (E)-caffeoyl-CoA→(R)-rosmarinate + coenzyme A (EC 2.3.1.140)
No genes
4-coumaroyl-4'-hydroxyphenyllactate + dioxygen + a reduced [NADPH-hemoprotein reductase]→caffeoyl-4'-hydroxyphenyllactate + an oxidized [NADPH-hemoprotein reductase] + H2O
No genes
4-coumaroyl-4'-hydroxyphenyllactate + dioxygen + a reduced [NADPH-hemoprotein reductase]→4-coumaroyl-3',4'-dihydroxyphenyllactate + an oxidized [NADPH-hemoprotein reductase] + H2O
No genes
caffeoyl-4'-hydroxyphenyllactate + dioxygen + a reduced [NADPH-hemoprotein reductase]→(R)-rosmarinate + an oxidized [NADPH-hemoprotein reductase] + H2O
No genes
4-coumaroyl-3',4'-dihydroxyphenyllactate + dioxygen + a reduced [NADPH-hemoprotein reductase]→(R)-rosmarinate + an oxidized [NADPH-hemoprotein reductase] + H2O
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information