MetaCyc Pathway: superpathway of histidine, purine, and pyrimidine biosynthesis in Pantoea agglomerans CFBP13505 P0401

MetaCyc Pathway: superpathway of histidine, purine, and pyrimidine biosynthesis in Pantoea agglomerans CFBP13505 P0401

Carbamoyl-phosphate synthase (glutamine-hydrolyzing):
L-glutamine + hydrogen carbonate + 2 ATP + H₂O→carbamoyl phosphate + 2 ADP + L-glutamate + 2 H⁺ + phosphate (EC 6.3.5.5)

PagCFBP13516_RS16425 (carB)

PagCFBP13516_RS16430 (carA)

Ribose-phosphate diphosphokinase:
D-ribose 5-phosphate + ATP→5-phospho-α-D-ribose 1-diphosphate + AMP + H⁺ (EC 2.7.6.1)

PagCFBP13516_RS11450 (prs)

Aspartate carbamoyltransferase:
carbamoyl phosphate + L-aspartate→N-carbamoyl-L-aspartate + H⁺ + phosphate (EC 2.1.3.2)

PagCFBP13516_RS17080 (pyrB)

PagCFBP13516_RS17085 (pyrI)

ATP phosphoribosyltransferase (in reverse):
5-phospho-α-D-ribose 1-diphosphate + ATP→1-(5-phospho-β-D-ribosyl)-ATP + diphosphate (EC 2.4.2.17)

PagCFBP13516_RS07480 (hisG)

Amidophosphoribosyltransferase (in reverse):
5-phospho-α-D-ribose 1-diphosphate + L-glutamine + H₂O→5-phospho-β-D-ribosylamine + L-glutamate + diphosphate (EC 2.4.2.14)

PagCFBP13516_RS06465 (purF)

Dihydroorotase (in reverse):
N-carbamoyl-L-aspartate + H⁺→(S)-dihydroorotate + H₂O (EC 3.5.2.3)

PagCFBP13516_RS02820

PagCFBP13516_RS12275 (pyrC)

Phosphoribosylamine--glycine ligase:
5-phospho-β-D-ribosylamine + ATP + glycine→N¹-(5-phospho-β-D-ribosyl)glycinamide + ADP + H⁺ + phosphate (EC 6.3.4.13)

PagCFBP13516_RS18290 (purD)

Phosphoribosyl-ATP diphosphatase:
1-(5-phospho-β-D-ribosyl)-ATP + H₂O→1-(5-phospho-β-D-ribosyl)-AMP + diphosphate + H⁺ (EC 3.6.1.31)

PagCFBP13516_RS07445 (hisIE)

Dihydroorotate dehydrogenase:
(S)-dihydroorotate_[in] + an electron-transfer quinone→orotate_[in] + an electron-transfer quinol (EC 1.3.5.2)

PagCFBP13516_RS12790 (pyrD)

N¹-(5-phospho-β-D-ribosyl)glycinamide + ATP + formate→N²-formyl-N¹-(5-phospho-β-D-ribosyl)glycinamide + ADP + H⁺ + phosphate (EC 6.3.1.21)

PagCFBP13516_RS08680 (purT)

Phosphoribosyl-AMP cyclohydrolase:
1-(5-phospho-β-D-ribosyl)-AMP + H₂O→1-(5-phospho-β-D-ribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide (EC 3.5.4.19)

PagCFBP13516_RS07445 (hisIE)

Phosphoribosylformylglycinamidine synthase:
N²-formyl-N¹-(5-phospho-β-D-ribosyl)glycinamide + L-glutamine + ATP + H₂O→2-(formamido)-N¹-(5-phospho-β-D-ribosyl)acetamidine + ADP + L-glutamate + H⁺ + phosphate (EC 6.3.5.3)

PagCFBP13516_RS05220 (purL)

Orotate phosphoribosyltransferase (in reverse):
orotate + 5-phospho-α-D-ribose 1-diphosphate→orotidine 5'-phosphate + diphosphate (EC 2.4.2.10)

PagCFBP13516_RS00590 (pyrE)

isomerase:
1-(5-phospho-β-D-ribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide→phosphoribulosylformimino-AICAR-phosphate (EC 5.3.1.16)

PagCFBP13516_RS07455 (hisA)

Phosphoribosylformylglycinamidine cyclo-ligase:
2-(formamido)-N¹-(5-phospho-β-D-ribosyl)acetamidine + ATP→5-amino-1-(5-phospho-β-D-ribosyl)imidazole + ADP + H⁺ + phosphate (EC 6.3.3.1)

PagCFBP13516_RS05470 (purM)

phosphoribulosylformimino-AICAR-phosphate + L-glutamine→5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)-glycerol 3-phosphate + L-glutamate + H⁺ (EC 4.3.2.10)

PagCFBP13516_RS07450 (hisF)

PagCFBP13516_RS07460 (hisH)

Orotidine-5'-phosphate decarboxylase:
orotidine 5'-phosphate + H⁺→UMP + CO₂ (EC 4.1.1.23)

PagCFBP13516_RS09410 (pyrF)

Imidazoleglycerol-phosphate dehydratase:
D-erythro-1-(imidazol-4-yl)-glycerol 3-phosphate→3-(imidazol-4-yl)-2-oxopropyl phosphate + H₂O (EC 4.2.1.19)

PagCFBP13516_RS07465 (hisB)

Cytidylate kinase:
UMP + ATP→UDP + ADP (EC 2.7.4.14; 2.7.4.22)

PagCFBP13516_RS12940 (cmk)

PagCFBP13516_RS15835 (pyrH)

5-(carboxyamino)imidazole ribonucleotide synthase:
5-amino-1-(5-phospho-β-D-ribosyl)imidazole + hydrogen carbonate + ATP→N⁵-carboxyaminoimidazole ribonucleotide + ADP + 2 H⁺ + phosphate (EC 6.3.4.18)

PagCFBP13516_RS14600 (purK)

Histidinol-phosphate transaminase (in reverse):
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate→L-histidinol phosphate + 2-oxoglutarate (EC 2.6.1.9)

PagCFBP13516_RS07470 (hisC)

5-(carboxyamino)imidazole ribonucleotide mutase:
N⁵-carboxyaminoimidazole ribonucleotide→5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate (EC 5.4.99.18)

PagCFBP13516_RS14595 (purE)

Nucleoside-diphosphate kinase:
UDP + ATP→UTP + ADP (EC 2.7.4.6)

PagCFBP13516_RS05325 (ndk)

PagCFBP13516_RS14695 (adk)

CTP synthase:
UTP + L-glutamine + ATP + H₂O→CTP + ADP + L-glutamate + 2 H⁺ + phosphate (EC 6.3.4.2)

PagCFBP13516_RS04400 (pyrG)

PagCFBP13516_RS10665

Histidinol-phosphatase:
L-histidinol phosphate + H₂O→histidinol + phosphate (EC 3.1.3.15)

PagCFBP13516_RS07465 (hisB)

Phosphoribosylaminoimidazolesuccinocarboxamide synthase:
L-aspartate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + ATP→5'-phosphoribosyl-4-(N-succinocarboxamide)-5-aminoimidazole + ADP + H⁺ + phosphate (EC 6.3.2.6)

PagCFBP13516_RS05565 (purC)

Adenylosuccinate lyase:
5'-phosphoribosyl-4-(N-succinocarboxamide)-5-aminoimidazole→5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + fumarate (EC 4.3.2.2)

PagCFBP13516_RS11980 (purB)

Histidinol dehydrogenase:
histidinol + NAD⁺→histidinal + NADH + H⁺ (EC 1.1.1.23)

PagCFBP13516_RS07475 (hisD)

Phosphoribosylaminoimidazolecarboxamide formyltransferase:
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + an N¹⁰-formyltetrahydrofolate→5-formamido-1-(5-phospho-D-ribosyl)-imidazole-4-carboxamide + a tetrahydrofolate (EC 2.1.2.3)

PagCFBP13516_RS18285 (purH)

Histidinol dehydrogenase:
histidinal + NAD⁺ + H₂O→L-histidine + NADH + 2 H⁺ (EC 1.1.1.23)

PagCFBP13516_RS07475 (hisD)

IMP cyclohydrolase (in reverse):
5-formamido-1-(5-phospho-D-ribosyl)-imidazole-4-carboxamide→IMP + H₂O (EC 3.5.4.10)

PagCFBP13516_RS18285 (purH)

Adenylosuccinate synthase:
IMP + L-aspartate + GTP→adenylosuccinate + GDP + 2 H⁺ + phosphate (EC 6.3.4.4)

PagCFBP13516_RS02630

IMP dehydrogenase:
IMP + NAD⁺ + H₂O→XMP + NADH + H⁺ (EC 1.1.1.205)

PagCFBP13516_RS05395 (guaB)

Adenylosuccinate lyase:
adenylosuccinate→AMP + fumarate (EC 4.3.2.2)

PagCFBP13516_RS11980 (purB)

GMP synthase (glutamine-hydrolyzing):
XMP + L-glutamine + ATP + H₂O→GMP + AMP + L-glutamate + diphosphate + 2 H⁺ (EC 6.3.5.2)

PagCFBP13516_RS05400 (guaA)

PagCFBP13516_RS11750

Adenylate kinase:
AMP + ATP→2 ADP (EC 2.7.4.3)

PagCFBP13516_RS14695 (adk)

Guanylate kinase:
GMP + ATP→GDP + ADP (EC 2.7.4.8)

PagCFBP13516_RS00505 (gmk)

PagCFBP13516_RS07965 (phnN)

Ribonucleoside-diphosphate reductase (in reverse):
ADP + a reduced thioredoxin→dADP + an oxidized thioredoxin + H₂O (EC 1.17.4.1)

PagCFBP13516_RS04910 (nrdF)

PagCFBP13516_RS04915 (nrdE)

PagCFBP13516_RS04920 (nrdI)

PagCFBP13516_RS06660 (nrdB)

PagCFBP13516_RS06665 (nrdA)

Nucleoside-diphosphate kinase:
GDP + ATP→GTP + ADP (EC 2.7.4.6)

PagCFBP13516_RS05325 (ndk)

PagCFBP13516_RS14695 (adk)

Ribonucleoside-diphosphate reductase (in reverse):
GDP + a reduced thioredoxin→dGDP + an oxidized thioredoxin + H₂O (EC 1.17.4.1)

PagCFBP13516_RS04910 (nrdF)

PagCFBP13516_RS04915 (nrdE)

PagCFBP13516_RS04920 (nrdI)

PagCFBP13516_RS06660 (nrdB)

PagCFBP13516_RS06665 (nrdA)

Ribonucleoside-diphosphate reductase (in reverse):
ADP + a reduced NrdH glutaredoxin-like protein→dADP + an oxidized NrdH glutaredoxin-like protein + H₂O (EC 1.17.4.1)

PagCFBP13516_RS04910 (nrdF)

PagCFBP13516_RS04915 (nrdE)

PagCFBP13516_RS06660 (nrdB)

PagCFBP13516_RS06665 (nrdA)

Ribonucleoside-diphosphate reductase (in reverse):
GDP + a reduced NrdH glutaredoxin-like protein→dGDP + an oxidized NrdH glutaredoxin-like protein + H₂O (EC 1.17.4.1)

PagCFBP13516_RS04910 (nrdF)

PagCFBP13516_RS04915 (nrdE)

PagCFBP13516_RS06660 (nrdB)

PagCFBP13516_RS06665 (nrdA)

Nucleoside-diphosphate kinase:
dADP + ATP→ADP + dATP (EC 2.7.4.6)

PagCFBP13516_RS05325 (ndk)

Nucleoside-diphosphate kinase:
dGDP + ATP→dGTP + ADP (EC 2.7.4.6)

PagCFBP13516_RS05325 (ndk)

PagCFBP13516_RS14695 (adk)

ATP + formate + H⁺→dATP + CO₂ + H₂O (EC 1.1.98.6)

PagCFBP13516_RS17145 (nrdD)

GTP + formate + H⁺→dGTP + CO₂ + H₂O (EC 1.1.98.6)

PagCFBP13516_RS17145 (nrdD)

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information