MetaCyc Pathway: pyruvate fermentation to isobutanol (engineered) in Serratia liquefaciens MT49

Add experiment(s):


Acetolactate synthase:
2 pyruvate + H+→(S)-2-acetolactate + CO2 (EC 2.2.1.6)
IAI46_03290
IAI46_03295
IAI46_03375
IAI46_03380
IAI46_17615
IAI46_18380
IAI46_21055
IAI46_24355
IAI46_24360
Ketol-acid reductoisomerase (in reverse):
(S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ (EC 1.1.1.86)
IAI46_24325
Dihydroxy-acid dehydratase:
(2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O (EC 4.2.1.9)
IAI46_03155
IAI46_24345
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
IAI46_02655
IAI46_07885
IAI46_12310
IAI46_14220
IAI46_19495
IAI46_21740
IAI46_22730
IAI46_22910

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information