MetaCyc Pathway: superpathway of L-threonine biosynthesis in Escherichia coli BW25113

Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)

Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)

Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H⁺→L-aspartate 4-semialdehyde + NADP⁺ + phosphate (EC 1.2.1.11)

b2319 (usg)

b3433 (asd)

Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H⁺→L-homoserine + NAD(P)⁺ (EC 1.1.1.3)

b0002 (thrA)

b3940 (metL)

Homoserine kinase:
L-homoserine + ATP→O-phospho-L-homoserine + ADP + H⁺ (EC 2.7.1.39)

b0003 (thrB)

Threonine synthase:
O-phospho-L-homoserine + H₂O→L-threonine + phosphate (EC 4.2.3.1)

b0004 (thrC)

Links:

Pathway details at MetaCyc
Fitness data for 9 genes
Select organism:
All MetaCyc pathways for Escherichia coli BW25113

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information