MetaCyc Pathway: L-asparagine biosynthesis III (tRNA-dependent) in Escherichia coli BW25113
| Glutamate synthase (NADPH): L-glutamine + H2O→ammonia + L-glutamate + H+ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7) |
| b0032 (carA) |
| b0033 (carB) |
| b0485 (ybaS) |
| b0674 (asnB) |
| b1263 (trpD) |
| b1264 (trpE) |
| b1524 (yneH) |
| b1812 (pabB) |
| b2468 (aegA) |
| b2507 (guaA) |
| b2611 (ypjD) |
| b2780 (pyrG) |
| b2887 (ygfT) |
| b3212 (gltB) |
| b3213 (gltD) |
| b3360 (pabA) |
| Aspartate--tRNA(Asn) ligase: L-aspartate + tRNAAsn + ATP→an L-aspartyl-[tRNAAsn] + AMP + diphosphate (EC 6.1.1.23) |
| No genes |
| Asparaginyl-tRNA synthase (glutamine-hydrolyzing): an L-aspartyl-[tRNAAsn] + ATP→a 4-phosphooxy-L-aspartyl-[tRNAAsn] + ADP (EC 6.3.5.6) |
| No genes |
| Asparaginyl-tRNA synthase (glutamine-hydrolyzing): a 4-phosphooxy-L-aspartyl-[tRNAAsn] + ammonia→an L-asparaginyl-[tRNAAsn] + phosphate (EC 6.3.5.6) |
| No genes |
Links:
- Pathway details at MetaCyc
- Fitness data for 16 genes
- All MetaCyc pathways for Escherichia coli BW25113
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information