MetaCyc Pathway: (S)-lactate fermentation to propanoate, acetate and hydrogen in Escherichia coli BW25113

Add experiment(s):


L-lactate dehydrogenase:
(S)-lactate + NAD+→pyruvate + NADH + H+ (EC 1.1.1.27)
b0306 (ykgE)
b0307 (ykgF)
Pyruvate carboxylase:
pyruvate + ATP + hydrogen carbonate→oxaloacetate + ADP + H+ + phosphate (EC 6.4.1.1)
No genes
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
b0823 (ybiW)
b0824 (ybiY)
b0902 (pflA)
b0903 (pflB)
b2579 (yfiD)
b3951 (pflD)
b3952 (pflC)
b3114 (tdcE)
formate + H+→H2 + CO2
b2483 (hyfC)
b2722 (hycD)
b2723 (hycC)
b2724 (hycB)
b4079 (fdhF)
Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
b0801 (ybiC)
b1479 (sfcA)
b3236 (mdh)
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
b2297 (pta)
b2458 (eutI)
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
b1849 (purT)
b2296 (ackA)
b2460 (eutQ)
b2461 (eutP)
b3115 (tdcD)
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2)
b1611 (fumC)
b1612 (fumA)
b1675 (ydhZ)
b2929 (yggD)
b4122 (fumB)
Succinate dehydrogenase (ubiquinone):
fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
b4151 (frdD)
b4152 (frdC)
b4153 (frdB)
b4154 (frdA)
succinate + propanoyl-CoA→succinyl-CoA + propanoate (EC 2.8.3.27)
b2920 (ygfH)
Methylmalonyl-CoA mutase (in reverse):
succinyl-CoA→(R)-methylmalonyl-CoA (EC 5.4.99.2)
b2917 (yliK)
Methylmalonyl-CoA epimerase:
(R)-methylmalonyl-CoA→(S)-methylmalonyl-CoA (EC 5.1.99.1)
No genes
(S)-methylmalonyl-CoA[in] + Na+[in] + H+[out]→propanoyl-CoA[in] + CO2[in] + Na+[out] (EC 7.2.4.3)
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information