MetaCyc Pathway: pyruvate fermentation to isobutanol (engineered) in Escherichia coli BW25113
| Acetolactate synthase: 2 pyruvate + H+→(S)-2-acetolactate + CO2 (EC 2.2.1.6) |
| b0078 (ilvH) |
| b3670 (ilvN) |
| b3671 (ilvB) |
| b3769 (ilvM) |
| b0077 (ilvI) |
| Ketol-acid reductoisomerase (in reverse): (S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ (EC 1.1.1.86) |
| b3774 (ilvC) |
| Dihydroxy-acid dehydratase: (2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O (EC 4.2.1.9) |
| b0269 (yagF) |
| b4297 (yjhG) |
| b3771 (ilvD) |
| Branched-chain-2-oxoacid decarboxylase: 3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72) |
| No genes |
| Alcohol dehydrogenase (in reverse): isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1) |
| b0325 (yahK) |
| b0356 (frmA) |
| b1241 (adhE) |
| b1478 (adhP) |
| b3253 (yhdH) |
| b4269 (yjgB) |
| b3589 (yiaY) |
Links:
- Pathway details at MetaCyc
- Fitness data for 16 genes
- All MetaCyc pathways for Escherichia coli BW25113
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information