MetaCyc Pathway: superpathway of histidine, purine, and pyrimidine biosynthesis in Escherichia coli BW25113

Add experiment(s):


Carbamoyl-phosphate synthase (glutamine-hydrolyzing):
L-glutamine + hydrogen carbonate + 2 ATP + H2O→carbamoyl phosphate + 2 ADP + L-glutamate + 2 H+ + phosphate (EC 6.3.5.5)
b0032 (carA)
b0033 (carB)
Ribose-phosphate diphosphokinase:
D-ribose 5-phosphate + ATP→5-phospho-α-D-ribose 1-diphosphate + AMP + H+ (EC 2.7.6.1)
b1207 (prsA)
Aspartate carbamoyltransferase:
carbamoyl phosphate + L-aspartate→N-carbamoyl-L-aspartate + H+ + phosphate (EC 2.1.3.2)
b4244 (pyrI)
b4245 (pyrB)
ATP phosphoribosyltransferase (in reverse):
5-phospho-α-D-ribose 1-diphosphate + ATP→1-(5-phospho-β-D-ribosyl)-ATP + diphosphate (EC 2.4.2.17)
b2019 (hisG)
Amidophosphoribosyltransferase (in reverse):
5-phospho-α-D-ribose 1-diphosphate + L-glutamine + H2O→5-phospho-β-D-ribosylamine + L-glutamate + diphosphate (EC 2.4.2.14)
b2312 (purF)
Dihydroorotase (in reverse):
N-carbamoyl-L-aspartate + H+→(S)-dihydroorotate + H2O (EC 3.5.2.3)
b1062 (pyrC)
Phosphoribosylamine--glycine ligase:
5-phospho-β-D-ribosylamine + ATP + glycine→N1-(5-phospho-β-D-ribosyl)glycinamide + ADP + H+ + phosphate (EC 6.3.4.13)
b4005 (purD)
Phosphoribosyl-ATP diphosphatase:
1-(5-phospho-β-D-ribosyl)-ATP + H2O→1-(5-phospho-β-D-ribosyl)-AMP + diphosphate + H+ (EC 3.6.1.31)
b2026 (hisI)
Dihydroorotate dehydrogenase:
(S)-dihydroorotate[in] + an electron-transfer quinone→orotate[in] + an electron-transfer quinol (EC 1.3.5.2)
b0945 (pyrD)
N1-(5-phospho-β-D-ribosyl)glycinamide + ATP + formate→N2-formyl-N1-(5-phospho-β-D-ribosyl)glycinamide + ADP + H+ + phosphate (EC 6.3.1.21)
b1849 (purT)
Phosphoribosyl-AMP cyclohydrolase:
1-(5-phospho-β-D-ribosyl)-AMP + H2O→1-(5-phospho-β-D-ribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide (EC 3.5.4.19)
b2026 (hisI)
Phosphoribosylformylglycinamidine synthase:
N2-formyl-N1-(5-phospho-β-D-ribosyl)glycinamide + L-glutamine + ATP + H2O→2-(formamido)-N1-(5-phospho-β-D-ribosyl)acetamidine + ADP + L-glutamate + H+ + phosphate (EC 6.3.5.3)
b2557 (purL)
Orotate phosphoribosyltransferase (in reverse):
orotate + 5-phospho-α-D-ribose 1-diphosphate→orotidine 5'-phosphate + diphosphate (EC 2.4.2.10)
b3642 (pyrE)
isomerase:
1-(5-phospho-β-D-ribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide→phosphoribulosylformimino-AICAR-phosphate (EC 5.3.1.16)
b2024 (hisA)
Phosphoribosylformylglycinamidine cyclo-ligase:
2-(formamido)-N1-(5-phospho-β-D-ribosyl)acetamidine + ATP→5-amino-1-(5-phospho-β-D-ribosyl)imidazole + ADP + H+ + phosphate (EC 6.3.3.1)
b2499 (purM)
phosphoribulosylformimino-AICAR-phosphate + L-glutamine→5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)-glycerol 3-phosphate + L-glutamate + H+ (EC 4.3.2.10)
b2023 (hisH)
b2025 (hisF)
Orotidine-5'-phosphate decarboxylase:
orotidine 5'-phosphate + H+→UMP + CO2 (EC 4.1.1.23)
b1281 (pyrF)
Imidazoleglycerol-phosphate dehydratase:
D-erythro-1-(imidazol-4-yl)-glycerol 3-phosphate→3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O (EC 4.2.1.19)
b2022 (hisB)
Cytidylate kinase:
UMP + ATP→UDP + ADP (EC 2.7.4.14; 2.7.4.22)
b0171 (pyrH)
b0910 (cmk)
5-(carboxyamino)imidazole ribonucleotide synthase:
5-amino-1-(5-phospho-β-D-ribosyl)imidazole + hydrogen carbonate + ATP→N5-carboxyaminoimidazole ribonucleotide + ADP + 2 H+ + phosphate (EC 6.3.4.18)
b0522 (purK)
Histidinol-phosphate transaminase (in reverse):
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate→L-histidinol phosphate + 2-oxoglutarate (EC 2.6.1.9)
b2021 (hisC)
5-(carboxyamino)imidazole ribonucleotide mutase:
N5-carboxyaminoimidazole ribonucleotide→5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate (EC 5.4.99.18)
b0523 (purE)
Nucleoside-diphosphate kinase:
UDP + ATP→UTP + ADP (EC 2.7.4.6)
b0474 (adk)
b2518 (ndk)
CTP synthase:
UTP + L-glutamine + ATP + H2O→CTP + ADP + L-glutamate + 2 H+ + phosphate (EC 6.3.4.2)
b2780 (pyrG)
Histidinol-phosphatase:
L-histidinol phosphate + H2O→histidinol + phosphate (EC 3.1.3.15)
b0383 (phoA)
b2022 (hisB)
Phosphoribosylaminoimidazolesuccinocarboxamide synthase:
L-aspartate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + ATP→5'-phosphoribosyl-4-(N-succinocarboxamide)-5-aminoimidazole + ADP + H+ + phosphate (EC 6.3.2.6)
b2476 (purC)
Adenylosuccinate lyase:
5'-phosphoribosyl-4-(N-succinocarboxamide)-5-aminoimidazole→5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + fumarate (EC 4.3.2.2)
b1131 (purB)
Histidinol dehydrogenase:
histidinol + NAD+→histidinal + NADH + H+ (EC 1.1.1.23)
b2020 (hisD)
Phosphoribosylaminoimidazolecarboxamide formyltransferase:
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + an N10-formyltetrahydrofolate→5-formamido-1-(5-phospho-D-ribosyl)-imidazole-4-carboxamide + a tetrahydrofolate (EC 2.1.2.3)
b4006 (purH)
Histidinol dehydrogenase:
histidinal + NAD+ + H2O→L-histidine + NADH + 2 H+ (EC 1.1.1.23)
b2020 (hisD)
IMP cyclohydrolase (in reverse):
5-formamido-1-(5-phospho-D-ribosyl)-imidazole-4-carboxamide→IMP + H2O (EC 3.5.4.10)
b4006 (purH)
Adenylosuccinate synthase:
IMP + L-aspartate + GTP→adenylosuccinate + GDP + 2 H+ + phosphate (EC 6.3.4.4)
b4177 (purA)
IMP dehydrogenase:
IMP + NAD+ + H2O→XMP + NADH + H+ (EC 1.1.1.205)
b2508 (guaB)
Adenylosuccinate lyase:
adenylosuccinate→AMP + fumarate (EC 4.3.2.2)
b1131 (purB)
GMP synthase (glutamine-hydrolyzing):
XMP + L-glutamine + ATP + H2O→GMP + AMP + L-glutamate + diphosphate + 2 H+ (EC 6.3.5.2)
b2507 (guaA)
Adenylate kinase:
AMP + ATP→2 ADP (EC 2.7.4.3)
b0474 (adk)
Guanylate kinase:
GMP + ATP→GDP + ADP (EC 2.7.4.8)
b3648 (gmk)
b4094 (phnN)
Ribonucleoside-diphosphate reductase (in reverse):
ADP + a reduced thioredoxin→dADP + an oxidized thioredoxin + H2O (EC 1.17.4.1)
b2234 (nrdA)
b2235 (nrdB)
b2674 (nrdI)
b2675 (nrdE)
b2676 (nrdF)
Nucleoside-diphosphate kinase:
GDP + ATP→GTP + ADP (EC 2.7.4.6)
b0474 (adk)
b2518 (ndk)
Ribonucleoside-diphosphate reductase (in reverse):
GDP + a reduced thioredoxin→dGDP + an oxidized thioredoxin + H2O (EC 1.17.4.1)
b2234 (nrdA)
b2235 (nrdB)
b2674 (nrdI)
b2675 (nrdE)
b2676 (nrdF)
Ribonucleoside-diphosphate reductase (in reverse):
ADP + a reduced NrdH glutaredoxin-like protein→dADP + an oxidized NrdH glutaredoxin-like protein + H2O (EC 1.17.4.1)
b2234 (nrdA)
b2235 (nrdB)
b2675 (nrdE)
b2676 (nrdF)
Ribonucleoside-diphosphate reductase (in reverse):
GDP + a reduced NrdH glutaredoxin-like protein→dGDP + an oxidized NrdH glutaredoxin-like protein + H2O (EC 1.17.4.1)
b2234 (nrdA)
b2235 (nrdB)
b2675 (nrdE)
b2676 (nrdF)
Nucleoside-diphosphate kinase:
dADP + ATP→ADP + dATP (EC 2.7.4.6)
b2518 (ndk)
Nucleoside-diphosphate kinase:
dGDP + ATP→dGTP + ADP (EC 2.7.4.6)
b0474 (adk)
b2518 (ndk)
ATP + formate + H+→dATP + CO2 + H2O (EC 1.1.98.6)
b4238 (nrdD)
GTP + formate + H+→dGTP + CO2 + H2O (EC 1.1.98.6)
b4238 (nrdD)

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information