MetaCyc Pathway: superpathway of N-acetylneuraminate degradation in Escherichia coli BW25113

Add experiment(s):


N-acetyl-β-neuraminate→aceneuramate (EC 5.1.3.24)
b3221 (yhcH)
N-acetylneuraminate lyase:
aceneuramate→N-acetyl-D-mannosamine + pyruvate (EC 4.1.3.3)
b3225 (nanA)
N-acylmannosamine kinase:
N-acetyl-D-mannosamine + ATP→N-acetyl-D-mannosamine 6-phosphate + ADP + H+ (EC 2.7.1.60)
b3222 (yhcI)
N-acylglucosamine-6-phosphate 2-epimerase:
N-acetyl-D-mannosamine 6-phosphate→N-acetyl-D-glucosamine 6-phosphate (EC 5.1.3.9)
b3223 (nanE)
N-acetylglucosamine-6-phosphate deacetylase:
N-acetyl-D-glucosamine 6-phosphate + H2O→D-glucosamine 6-phosphate + acetate (EC 3.5.1.25)
b0677 (nagA)
Glucosamine-6-phosphate deaminase:
D-glucosamine 6-phosphate + H2O→β-D-fructofuranose 6-phosphate + ammonium (EC 3.5.99.6)
b0678 (nagB)
6-phosphofructokinase:
β-D-fructofuranose 6-phosphate + ATP→β-D-fructofuranose 1,6-bisphosphate + ADP + H+ (EC 2.7.1.11)
b1723 (pfkB)
b3916 (pfkA)
Fructose-bisphosphate aldolase:
β-D-fructofuranose 1,6-bisphosphate→glycerone phosphate + D-glyceraldehyde 3-phosphate (EC 4.1.2.13)
b2097 (b2097)
b2925 (fbaA)
Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H2O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)
b0383 (phoA)
b0766 (ybhA)
b2930 (yggF)
b3925 (glpX)
b4232 (fbp)
Triose-phosphate isomerase (in reverse):
glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)
b3919 (tpiA)
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
b1779 (gapA)
Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
b2926 (pgk)
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
b0755 (gpmA)
b3612 (gpmI)
b4395 (ytjC)
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.11)
b0755 (gpmA)
Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
b2779 (eno)
Pyruvate, water dikinase:
pyruvate + ATP + H2O→phosphoenolpyruvate + AMP + 2 H+ + phosphate (EC 2.7.9.2)
b1702 (pps)
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
b1676 (pykF)
b1854 (pykA)
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
b0823 (ybiW)
b0824 (ybiY)
b0902 (pflA)
b0903 (pflB)
b2579 (yfiD)
b3951 (pflD)
b3952 (pflC)
b3114 (tdcE)
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
b0351 (mhpF)
b1241 (adhE)
b2455 (eutE)
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
b2297 (pta)
b2458 (eutI)
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
b1849 (purT)
b2296 (ackA)
b2460 (eutQ)
b2461 (eutP)
b3115 (tdcD)
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
b0325 (yahK)
b0356 (frmA)
b1241 (adhE)
b1478 (adhP)
b3253 (yhdH)
b4269 (yjgB)
b3589 (yiaY)

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information