MetaCyc Pathway: superpathway of Kdo2-lipid A biosynthesis in Escherichia coli BW25113

Add experiment(s):


Arabinose-5-phosphate isomerase (in reverse):
D-ribulose 5-phosphate→aldehydo-D-arabinose 5-phosphate (EC 5.3.1.13)
b2708 (gutQ)
b3197 (kdsD)
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase:
a (3R)-3-hydroxytetradecanoyl-[acp] + UDP-N-acetyl-α-D-glucosamine→UDP-3-O-[(3R)-3-hydroxymyristoyl]-N-acetyl-α-D-glucosamine + a soluble [acyl-carrier protein] (EC 2.3.1.129)
b0181 (lpxA)
aldehydo-D-arabinose 5-phosphate→D-arabinofuranose 5-phosphate (spontaneous)
No genes
UDP-3-O-[(3R)-3-hydroxymyristoyl]-N-acetyl-α-D-glucosamine + H2O→UDP-3-O-[(3R)-3-hydroxymyristoyl]-α-D-glucosamine + acetate (EC 3.5.1.108)
b0096 (lpxC)
3-deoxy-8-phosphooctulonate synthase:
D-arabinofuranose 5-phosphate + phosphoenolpyruvate + H2O→3-deoxy-D-manno-octulosonate 8-phosphate + phosphate (EC 2.5.1.55)
b1215 (kdsA)
a (3R)-3-hydroxytetradecanoyl-[acp] + UDP-3-O-[(3R)-3-hydroxymyristoyl]-α-D-glucosamine→UDP-2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-α-D-glucosamine + a soluble [acyl-carrier protein] + H+ (EC 2.3.1.191)
b0179 (lpxD)
3-deoxy-manno-octulosonate-8-phosphatase:
3-deoxy-D-manno-octulosonate 8-phosphate + H2O→3-deoxy-α-D-manno-2-octulosonate + phosphate (EC 3.1.3.45)
b3198 (kdsC)
UDP-2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-α-D-glucosamine + H2O→lipid X (E. coli) + 2 H+ + UMP (EC 3.6.1.54)
b0524 (lpxH)
3-deoxy-manno-octulosonate cytidylyltransferase:
3-deoxy-α-D-manno-2-octulosonate + CTP→CMP-3-deoxy-β-D-manno-octulosonate + diphosphate (EC 2.7.7.38)
b0918 (kdsB)
Lipid-A-disaccharide synthase:
lipid X (E. coli) + UDP-2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-α-D-glucosamine→lipid A disaccharide (E. coli) + H+ + UDP (EC 2.4.1.182)
b0182 (lpxB)
Tetraacyldisaccharide 4'-kinase:
lipid A disaccharide (E. coli) + ATP→lipid IVA (E. coli) + ADP + H+ (EC 2.7.1.130)
b0915 (lpxK)
CMP-3-deoxy-β-D-manno-octulosonate + lipid IVA (E. coli)→α-Kdo-(2→6)-lipid IVA (E. coli) + CMP + H+ (EC 2.4.99.12)
b3633 (kdtA)
CMP-3-deoxy-β-D-manno-octulosonate + α-Kdo-(2→6)-lipid IVA (E. coli)→α-Kdo-(2->4)-α-Kdo-(2->6)-lipid IVA (E. coli) + CMP + H+ (EC 2.4.99.13)
b3633 (kdtA)
α-Kdo-(2->4)-α-Kdo-(2->6)-lipid IVA (E. coli) + a dodecanoyl-[acp]→α-Kdo-(2->4)-α-Kdo-(2->6)-(lauroyl)-lipid IVA (E. coli) + a soluble [acyl-carrier protein] (EC 2.3.1.241)
b1054 (lpxL)
α-Kdo-(2->4)-α-Kdo-(2->6)-(lauroyl)-lipid IVA (E. coli) + a myristoyl-[acp]→α-D-Kdo-(2→4)-α-D-Kdo-(2→6)-lipid A (E. coli) + a soluble [acyl-carrier protein] (EC 2.3.1.243)
b1855 (lpxM)
α-D-Kdo-(2→4)-α-D-Kdo-(2→6)-lipid A (E. coli) + ADP-L-glycero-β-D-manno-heptose→α-Hep-(1→5)-[α-Kdo-(2→4)]-α-Kdo-(2→6)-lipid A (E. coli) + ADP + H+ (EC 2.4.99.23)
b3621 (rfaC)
ADP-L-glycero-β-D-manno-heptose + α-Hep-(1→5)-[α-Kdo-(2→4)]-α-Kdo-(2→6)-lipid A (E. coli)→α-Hep-(1→3)-α-Hep-(1→5)-[α-Kdo-(2→4)]-α-Kdo-(2→6)-lipid A (E. coli) + ADP + H+ (EC 2.4.99.24)
b3620 (rfaF)
α-Hep-(1→3)-α-Hep-(1→5)-[α-Kdo-(2→4)]-α-Kdo-(2→6)-lipid A (E. coli) + ATP→α-Hep-(1→3)-4-O-phospho-α-Hep-(1→5)-[α-Kdo-(2→4)]-α-Kdo-(2→6)-lipid A (E. coli) + ADP + H+ (EC 2.7.1.235)
b3630 (rfaP)
ADP-L-glycero-β-D-manno-heptose + α-Hep-(1→3)-4-O-phospho-α-Hep-(1→5)-[α-Kdo-(2→4)]-α-Kdo-(2→6)-lipid A (E. coli)→(heptosyl)3-Kdo2-lipid A-phosphate (E. coli) + ADP + H+ (EC 2.4.99.25)
b3632 (rfaQ)
(heptosyl)3-Kdo2-lipid A-phosphate (E. coli) + UDP-α-D-glucose→glucosyl-(heptosyl)3-Kdo2-lipid A-phosphate (E. coli) + H+ + UDP (EC 2.4.1.M73)
b3631 (rfaG)
glucosyl-(heptosyl)3-Kdo2-lipid A-phosphate (E. coli) + ATP→glucosyl-(heptosyl)3-Kdo2-lipid A-bisphosphate (E. coli) + ADP + H+
b3625 (rfaY)
glucosyl-(heptosyl)3-Kdo2-lipid A-bisphosphate (E. coli) + UDP-α-D-galactose→galactosyl-(1→6)-glucosyl-(1→3)-(heptosyl)3-Kdo2-lipid A-bisphosphate (E. coli) + H+ + UDP
b3628 (rfaB)
Lipopolysaccharide glucosyltransferase II:
UDP-α-D-glucose + galactosyl-(1→6)-glucosyl-(1→3)-(heptosyl)3-Kdo2-lipid A-bisphosphate (E. coli)→galactosyl-(glucosyl)2-(heptosyl)3-Kdo2-lipid A-bisphosphate (E. coli) + H+ + UDP (EC 2.4.1.73)
b3627 (rfaI)
Lipopolysaccharide glucosyltransferase I:
UDP-α-D-glucose + galactosyl-(glucosyl)2-(heptosyl)3-Kdo2-lipid A-bisphosphate (E. coli)→galactosyl-(glucosyl)3-(heptosyl)3-Kdo2-lipid A-bisphosphate (E. coli) + H+ + UDP (EC 2.4.1.58)
b3626 (rfaJ)
ADP-L-glycero-β-D-manno-heptose + galactosyl-(glucosyl)3-(heptosyl)3-Kdo2-lipid A-bisphosphate (E. coli)→lipid A-core oligosaccharide (E. coli K-12 core type) + ADP + H+ (EC 2.4.1.M47)
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information