MetaCyc Pathway: mixed acid fermentation in Escherichia coli BW25113

Add experiment(s):


Phosphoenolpyruvate carboxylase (in reverse):
phosphoenolpyruvate + hydrogen carbonate→oxaloacetate + phosphate (EC 4.1.1.31)
b3956 (ppc)
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
b1676 (pykF)
b1854 (pykA)
D-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(R)-lactate + NAD+ (EC 1.1.1.28)
b1380 (ldhA)
b2133 (dld)
Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
b0801 (ybiC)
b1479 (sfcA)
b3236 (mdh)
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
b0823 (ybiW)
b0824 (ybiY)
b0902 (pflA)
b0903 (pflB)
b2579 (yfiD)
b3951 (pflD)
b3952 (pflC)
b3114 (tdcE)
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
b0351 (mhpF)
b1241 (adhE)
b2455 (eutE)
Citrate (Si)-synthase:
acetyl-CoA + oxaloacetate + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
b0720 (gltA)
formate + H+→CO2 + H2
b2483 (hyfC)
b2722 (hycD)
b2723 (hycC)
b2724 (hycB)
b4079 (fdhF)
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2)
b1611 (fumC)
b1612 (fumA)
b1675 (ydhZ)
b2929 (yggD)
b4122 (fumB)
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
b2297 (pta)
b2458 (eutI)
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
b1849 (purT)
b2296 (ackA)
b2460 (eutQ)
b2461 (eutP)
b3115 (tdcD)
Aconitate hydratase:
citrate→cis-aconitate + H2O (EC 4.2.1.3)
b0118 (acnB)
b0771 (ybhJ)
b1276 (acnA)
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
b0325 (yahK)
b0356 (frmA)
b1241 (adhE)
b1478 (adhP)
b3253 (yhdH)
b4269 (yjgB)
b3589 (yiaY)
Succinate dehydrogenase (ubiquinone):
fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
b4151 (frdD)
b4152 (frdC)
b4153 (frdB)
b4154 (frdA)
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
b0118 (acnB)
b0771 (ybhJ)
b1276 (acnA)
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→CO2 + 2-oxoglutarate + NADPH (EC 1.1.1.42)
b1136 (icd)

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information