MetaCyc Pathway: superpathway of branched chain amino acid biosynthesis in Escherichia coli BW25113

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Acetolactate synthase:
2 pyruvate + H+→(S)-2-acetolactate + CO2 (EC 2.2.1.6)
b0078 (ilvH)
b3670 (ilvN)
b3671 (ilvB)
b3769 (ilvM)
b0077 (ilvI)
Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
b3117 (tdcB)
b3772 (ilvA)
Ketol-acid reductoisomerase (in reverse):
(S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ (EC 1.1.1.86)
b3774 (ilvC)
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
b3117 (tdcB)
b3772 (ilvA)
Dihydroxy-acid dehydratase:
(2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O (EC 4.2.1.9)
b0269 (yagF)
b4297 (yjhG)
b3771 (ilvD)
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
b3113 (yhaR)
b3117 (tdcB)
b3772 (ilvA)
b4243 (yjgF)
2-isopropylmalate synthase:
3-methyl-2-oxobutanoate + acetyl-CoA + H2O→(2S)-2-isopropylmalate + coenzyme A + H+ (EC 2.3.3.13)
b0074 (leuA)
Acetolactate synthase:
2-oxobutanoate + pyruvate + H+→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
b0078 (ilvH)
b3670 (ilvN)
b3671 (ilvB)
b3769 (ilvM)
b0077 (ilvI)
Branched-chain-amino-acid transaminase (in reverse):
3-methyl-2-oxobutanoate + L-glutamate→L-valine + 2-oxoglutarate (EC 2.6.1.42)
b3770 (ilvE)
3-isopropylmalate dehydratase (in reverse):
(2S)-2-isopropylmalate→2-isopropylmaleate + H2O (EC 4.2.1.33)
b0071 (leuD)
b0072 (leuC)
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
b3774 (ilvC)
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
b0269 (yagF)
b4297 (yjhG)
b3771 (ilvD)
3-isopropylmalate dehydratase (in reverse):
2-isopropylmaleate + H2O→(2R,3S)-3-isopropylmalate (EC 4.2.1.33)
b0071 (leuD)
b0072 (leuC)
3-isopropylmalate dehydrogenase:
(2R,3S)-3-isopropylmalate + NAD+→(2S)-2-isopropyl-3-oxosuccinate + NADH + H+ (EC 1.1.1.85)
b0073 (leuB)
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
b3770 (ilvE)
3-isopropylmalate dehydrogenase:
(2S)-2-isopropyl-3-oxosuccinate + H+→4-methyl-2-oxopentanoate + CO2 (spontaneous) (EC 1.1.1.85)
b0073 (leuB)
Branched-chain-amino-acid transaminase (in reverse):
4-methyl-2-oxopentanoate + L-glutamate→L-leucine + 2-oxoglutarate (EC 2.6.1.42; 2.6.1.6)
b4054 (tyrB)
b3770 (ilvE)

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information