MetaCyc Pathway: pyruvate fermentation to butanol II (engineered) in Enterobacter asburiae PDN3

Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)

3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H⁺→(S)-3-hydroxybutanoyl-CoA + NAD⁺ (EC 1.1.1.35)

EX28DRAFT_0271

EX28DRAFT_4510

(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H₂O (EC 4.2.1.150)

EX28DRAFT_0271

EX28DRAFT_0959

EX28DRAFT_4510

Trans-2-enoyl-CoA reductase (NAD(+)) (in reverse):
crotonyl-CoA + NADH + H⁺→butanoyl-CoA + NAD⁺ (EC 1.3.1.44)

EX28DRAFT_2668

Butanal dehydrogenase (in reverse):
butanoyl-CoA + NAD(P)H + H⁺→1-butanal + coenzyme A + NAD(P)⁺ (EC 1.2.1.57)

No genes

1-butanal + NADH + H⁺→butan-1-ol + NAD⁺

No genes

Links:

Pathway details at MetaCyc
Fitness data for 9 genes
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All MetaCyc pathways for Enterobacter asburiae PDN3

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information