MetaCyc Pathway: N⁶-L-threonylcarbamoyladenosine³⁷-modified tRNA biosynthesis in Escherichia coli HS(pFamp)R (ATCC 700891)

L-threonine + ATP + hydrogen carbonate→(L-threonylcarbamoyl)adenylate + diphosphate + H2O (EC 2.7.7.87)

OHPLBJKB_00421

(L-threonylcarbamoyl)adenylate + an adenine37 in tRNA→an N6-L-threonylcarbamoyladenine37 in tRNA + AMP + H+ (EC 2.3.1.234)

OHPLBJKB_00638

OHPLBJKB_01926

OHPLBJKB_03874

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information