MetaCyc Pathway: benzoyl-CoA biosynthesis in Escherichia coli HS(pFamp)R (ATCC 700891)

Enoyl-CoA hydratase (in reverse):
(E)-cinnamoyl-CoA + H₂O→(S)-3-hydroxy-3-phenylpropanoyl-CoA (EC 4.2.1.17)

3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxy-3-phenylpropanoyl-CoA + NAD⁺→3-oxo-3-phenylpropanoyl-CoA + NADH + H⁺ (EC 1.1.1.35)

OHPLBJKB_01365

OHPLBJKB_04207

Acetyl-CoA C-acyltransferase (in reverse):
3-oxo-3-phenylpropanoyl-CoA + coenzyme A→benzoyl-CoA + acetyl-CoA (EC 2.3.1.16)

Links:

Pathway details at MetaCyc
Fitness data for 9 genes
Select organism:
All MetaCyc pathways for Escherichia coli HS(pFamp)R (ATCC 700891)

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information