MetaCyc Pathway: superpathway of L-methionine biosynthesis (transsulfuration) in Escherichia coli HS(pFamp)R (ATCC 700891)

MetaCyc Pathway: superpathway of L-methionine biosynthesis (transsulfuration) in Escherichia coli HS(pFamp)R (ATCC 700891)

Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)

Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)

Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H⁺→L-aspartate 4-semialdehyde + NADP⁺ + phosphate (EC 1.2.1.11)

Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H⁺→L-homoserine + NAD(P)⁺ (EC 1.1.1.3)

Homoserine O-succinyltransferase:
L-homoserine + succinyl-CoA→O-succinyl-L-homoserine + coenzyme A (EC 2.3.1.46)

Cystathionine gamma-synthase:
O-succinyl-L-homoserine + L-cysteine→L-cystathionine + H⁺ + succinate (EC 2.5.1.48)

Cysteine-S-conjugate beta-lyase:
L-cystathionine + H₂O→L-homocysteine + ammonium + pyruvate (EC 4.4.1.13)

5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase:
L-homocysteine + 5-methyltetrahydropteroyl tri-L-glutamate→L-methionine + tetrahydropteroyl tri-L-glutamate (EC 2.1.1.14)

Methionine synthase:
L-homocysteine + a 5-methyltetrahydrofolate→L-methionine + a tetrahydrofolate (EC 2.1.1.13)

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information