MetaCyc Pathway: L-isoleucine degradation II in Escherichia coli HS(pFamp)R (ATCC 700891)

Branched-chain-amino-acid transaminase:
L-isoleucine + 2-oxoglutarate→(3S)-3-methyl-2-oxopentanoate + L-glutamate (EC 2.6.1.42)

OHPLBJKB_04283

Branched-chain-2-oxoacid decarboxylase:
(3S)-3-methyl-2-oxopentanoate + H⁺→2-methylbutanal + CO₂ (EC 4.1.1.72)

No genes

Alcohol dehydrogenase (in reverse):
2-methylbutanal + NADH + H⁺→2-methylbutanol + NAD⁺ (EC 1.1.1.1)

Links:

Pathway details at MetaCyc
Fitness data for 9 genes
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All MetaCyc pathways for Escherichia coli HS(pFamp)R (ATCC 700891)

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information