MetaCyc Pathway: formaldehyde assimilation III (dihydroxyacetone cycle) in Escherichia coli HS(pFamp)R (ATCC 700891)

MetaCyc Pathway: formaldehyde assimilation III (dihydroxyacetone cycle) in Escherichia coli HS(pFamp)R (ATCC 700891)

Transketolase:
D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate→D-ribose 5-phosphate + D-xylulose 5-phosphate (EC 2.2.1.1)

Ribose-5-phosphate isomerase:
D-ribose 5-phosphate→D-ribulose 5-phosphate (EC 5.3.1.6)

Ribulose-phosphate 3-epimerase:
D-ribulose 5-phosphate→D-xylulose 5-phosphate (EC 5.1.3.1)

Formaldehyde transketolase:
D-xylulose 5-phosphate + formaldehyde→D-glyceraldehyde 3-phosphate + dihydroxyacetone (EC 2.2.1.3)

No genes

Glycerone kinase:
dihydroxyacetone + ATP→glycerone phosphate + ADP + H⁺ (EC 2.7.1.29)

No genes

Fructose-bisphosphate aldolase (in reverse):
glycerone phosphate + D-glyceraldehyde 3-phosphate→β-D-fructofuranose 1,6-bisphosphate (EC 4.1.2.13)

Triose-phosphate isomerase (in reverse):
glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)

Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H₂O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)

Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD⁺ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H⁺ (EC 1.2.1.12)

Transketolase (in reverse):
β-D-fructofuranose 6-phosphate + D-glyceraldehyde 3-phosphate→D-xylulose 5-phosphate + D-erythrose 4-phosphate (EC 2.2.1.1)

Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)

Transaldolase (in reverse):
β-D-fructofuranose 6-phosphate + D-erythrose 4-phosphate→D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate (EC 2.2.1.2)

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information