MetaCyc Pathway: heterolactic fermentation in Escherichia coli HS(pFamp)R (ATCC 700891)

MetaCyc Pathway: heterolactic fermentation in Escherichia coli HS(pFamp)R (ATCC 700891)

Fructokinase:
β-D-fructofuranose + ATP→β-D-fructofuranose 6-phosphate + ADP + H⁺ (EC 2.7.1.4)

Hexokinase:
D-glucopyranose + ATP→D-glucopyranose 6-phosphate + ADP + H⁺ (EC 2.7.1.1; 2.7.1.2)

Glucose-6-phosphate isomerase (in reverse):
β-D-fructofuranose 6-phosphate→α-D-glucose 6-phosphate (EC 5.3.1.9)

Glucose-6-phosphate dehydrogenase:
D-glucopyranose 6-phosphate + NADP⁺→6-phospho D-glucono-1,5-lactone + NADPH + H⁺ (EC 1.1.1.49)

6-phosphogluconolactonase:
6-phospho D-glucono-1,5-lactone + H₂O→D-gluconate 6-phosphate + H⁺ (EC 3.1.1.31)

D-gluconate 6-phosphate + NAD(P)⁺→CO₂ + D-ribulose 5-phosphate + NAD(P)H (EC 1.1.1.351)

No genes

Ribulose-phosphate 3-epimerase:
D-ribulose 5-phosphate→D-xylulose 5-phosphate (EC 5.1.3.1)

Phosphoketolase:
D-xylulose 5-phosphate + phosphate→acetyl phosphate + D-glyceraldehyde 3-phosphate + H₂O (EC 4.1.2.9)

No genes

Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD⁺ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H⁺ (EC 1.2.1.12)

Phosphate acetyltransferase (in reverse):
acetyl phosphate + coenzyme A→acetyl-CoA + phosphate (EC 2.3.1.8)

Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H⁺→acetaldehyde + coenzyme A + NAD⁺ (EC 1.2.1.10)

Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)

Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H⁺→ethanol + NAD⁺ (EC 1.1.1.1)

3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.11)

Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H₂O (EC 4.2.1.11)

Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H⁺→pyruvate + ATP (EC 2.7.1.40)

D-lactate dehydrogenase (in reverse):
pyruvate + NADH + H⁺→(R)-lactate + NAD⁺ (EC 1.1.1.28)

L-lactate dehydrogenase (in reverse):
pyruvate + NADH + H⁺→(S)-lactate + NAD⁺ (EC 1.1.1.27)

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information