MetaCyc Pathway: superpathway of L-threonine biosynthesis in Escherichia coli ECRC98

MetaCyc Pathway: superpathway of L-threonine biosynthesis in Escherichia coli ECRC98

Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)

JDDGAC_04070 (ydcR)

JDDGAC_08995 (aspC)

JDDGAC_15045 (yjiR)

JDDGAC_24515 (aRO8)

Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)

JDDGAC_14705 (thrA)

JDDGAC_16805 (lysC)

JDDGAC_17295 (metL)

Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H⁺→L-aspartate 4-semialdehyde + NADP⁺ + phosphate (EC 1.2.1.11)

JDDGAC_20505 (asd)

JDDGAC_26625 (usg)

Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H⁺→L-homoserine + NAD(P)⁺ (EC 1.1.1.3)

JDDGAC_14705 (thrA)

JDDGAC_17295 (metL)

Homoserine kinase:
L-homoserine + ATP→O-phospho-L-homoserine + ADP + H⁺ (EC 2.7.1.39)

JDDGAC_14700 (thrB)

Threonine synthase:
O-phospho-L-homoserine + H₂O→L-threonine + phosphate (EC 4.2.3.1)

JDDGAC_14695 (thrC)

Links:

Pathway details at MetaCyc
Fitness data for 11 genes
Select organism:
All MetaCyc pathways for Escherichia coli ECRC98

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information