MetaCyc Pathway: pyruvate fermentation to isobutanol (engineered) in Escherichia coli ECRC98

Add experiment(s):


Acetolactate synthase:
2 pyruvate + H+→(S)-2-acetolactate + CO2 (EC 2.2.1.6)
JDDGAC_14290 (ilvN)
JDDGAC_14295 (ilvI)
JDDGAC_18270
JDDGAC_18275 (ilvG)
JDDGAC_18775
JDDGAC_18780 (ilvN)
Ketol-acid reductoisomerase (in reverse):
(S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ (EC 1.1.1.86)
JDDGAC_18245 (ilvC)
Dihydroxy-acid dehydratase:
(2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O (EC 4.2.1.9)
JDDGAC_18260 (ilvD)
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
JDDGAC_03855 (adhP)
JDDGAC_05970 (adhE)
JDDGAC_12395 (frmA)
JDDGAC_12580 (yahK)
JDDGAC_15410 (ahr)
JDDGAC_19485 (yiaY)
JDDGAC_21340 (acuI)

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information